Product Name: KinSub1DDLYG
Product Number: PE-01AGQ95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1DDLYG
Product Use: For assaying the phosphotransferase activity of Tyrosine-protein kinase Hck (Hck, UniProt ID P08631). The KinSub1DDLYG peptide demonstrated very high phosphotransferase activity with Blk, and exhibited medium specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1DDLYG was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: EGLEDDLYGGPGPGG
Product Use: For assaying the phosphotransferase activity of Tyrosine-protein kinase Hck (Hck, UniProt ID P08631). The KinSub1DDLYG peptide demonstrated very high phosphotransferase activity with Blk, and exhibited medium specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1DDLYG was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: EGLEDDLYGGPGPGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1431.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Blk
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1431.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Blk
Scientific Background: Hck is one of several protein kinases that can phosphorylate KinSub1DDLYG. Human Hck is a protein-tyrosine kinase of 526 amino acid length, with a predicted molecular mass of 59,600 Da. It is a member of the TK group of protein kinases in the Src family. This kinase is highly expressed and widely distributed in most tested human tissues. Hck is especially expressed in certain hemopoietic cells and especially prominent in cells of myeloid lineage, particularly mature granulocytes and monocytes (1). Orthologues of Hck are highly conserved in vertebrates, including amphibians. Hck is inhibited by phosphorylation at Y522. The Hck gene is located on chromosome sequence 20q11-q12, a region that is affected by interstitial deletions in some acute myeloid leukemias and myeloproliferative disorders, which indicates that damage to Hck may contribute to the pathogenesis of these conditions (2). Hck has also been linked with the development of ovarian mucinous carcinomas.
References
[1] Ziegler SF, Marth JD, Lewis DB, Perlmutter RM. Novel protein-tyrosine kinase gene (hck) preferentially expressed in cells of hematopoietic origin. Mol Cell Biol. 1987 Jun;7(6):2276-85. PMID: 3453117
[2] Quintrell N, Lebo R, Varmus H, Bishop JM, Pettenati MJ, Le Beau MM, Diaz MO, Rowley JD. Identification of a human gene (Hck) that encodes a protein-tyrosine kinase and is expressed in hemopoietic cells. Mol Cell Biol. 1987 Jun;7(6):2267-75. PMID: 3496523
[1] Ziegler SF, Marth JD, Lewis DB, Perlmutter RM. Novel protein-tyrosine kinase gene (hck) preferentially expressed in cells of hematopoietic origin. Mol Cell Biol. 1987 Jun;7(6):2276-85. PMID: 3453117
[2] Quintrell N, Lebo R, Varmus H, Bishop JM, Pettenati MJ, Le Beau MM, Diaz MO, Rowley JD. Identification of a human gene (Hck) that encodes a protein-tyrosine kinase and is expressed in hemopoietic cells. Mol Cell Biol. 1987 Jun;7(6):2267-75. PMID: 3496523
© Kinexus Bioinformatics Corporation 2017