Product Name: KinSub1DDLYV
Product Number: PE-01AGS95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1DDLYV
Product Use: For assaying the phosphotransferase activity of C-terminus of Src tyrosine kinase (CSK, UniProt ID P41240). The KinSub1DDLYV peptide demonstrated high phosphotransferase activity with Brk, and exhibited moderate specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1DDLYV was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGLEDDLYVYFGGGG
Product Use: For assaying the phosphotransferase activity of C-terminus of Src tyrosine kinase (CSK, UniProt ID P41240). The KinSub1DDLYV peptide demonstrated high phosphotransferase activity with Brk, and exhibited moderate specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1DDLYV was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGLEDDLYVYFGGGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1517.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Brk
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1517.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Brk
Scientific Background: CSK is one of several protein kinases that can phosphorylate KinSub1DDLYV. Human Csk is a protein-tyrosine kinase of 450 amino acid length, with a predicted molecular mass of 50,704 Da. It is a member of the TK group of protein kinases in the Csk family. This kinase is moderate to highly expressed in most tested human tissues except apparently in the brain and spinal cord. Orthologues of Csk are highly conserved in vertebrates and insects. Csk is activated by phosphorylation at Y184, Y304 and S364. Csk has been shown to downregulate the tyrosine kinase activity of the Src through tyrosine phosphorylation of the Src carboxy terminus (1). A yeast 2-hybrid system has been used to identify proteins associated with Csk. The Src homology-3 (SH3) domain of CSK associates with a proline-rich region of PEP, a protein-tyrosine phosphatase expressed in hemopoietic cells (2). This association is highly specific and it is speculated that PEP may be an effector and/or regulator of CSK in T cells and other hemopoietic cells.
References
[1] Partanen J, Armstrong E, Bergman M, Mäkelä TP, Hirvonen H, Huebner K, Alitalo K. cyl encodes a putative cytoplasmic tyrosine kinase lacking the conserved tyrosine autophosphorylation site (Y416src). Oncogene. 1991 Nov;6(11):2013-8. PMID: 1945408.
[2] Cloutier JF, Veillette A. Association of inhibitory tyrosine protein kinase p50csk with protein tyrosine phosphatase PEP in T cells and other hemopoietic cells. EMBO J. 1996 Sep 16;15(18):4909-18. PMID: 8890164.
[1] Partanen J, Armstrong E, Bergman M, Mäkelä TP, Hirvonen H, Huebner K, Alitalo K. cyl encodes a putative cytoplasmic tyrosine kinase lacking the conserved tyrosine autophosphorylation site (Y416src). Oncogene. 1991 Nov;6(11):2013-8. PMID: 1945408.
[2] Cloutier JF, Veillette A. Association of inhibitory tyrosine protein kinase p50csk with protein tyrosine phosphatase PEP in T cells and other hemopoietic cells. EMBO J. 1996 Sep 16;15(18):4909-18. PMID: 8890164.
© Kinexus Bioinformatics Corporation 2017