Product Name: KinSub1DDNYV
Product Number: PE-01AGU95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1DDNYV
Product Use: For assaying the phosphotransferase activity of Platelet-derived growth factor receptor kinase alpha (PDGFRA, UniProt ID P16234). The KinSub1DDNYV peptide demonstrated very high phosphotransferase activity with Blk, and exhibited medium specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1DDNYV was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGGEDDNYVGVGGGG
Product Use: For assaying the phosphotransferase activity of Platelet-derived growth factor receptor kinase alpha (PDGFRA, UniProt ID P16234). The KinSub1DDNYV peptide demonstrated very high phosphotransferase activity with Blk, and exhibited medium specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1DDNYV was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGGEDDNYVGVGGGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1308.3 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Blk
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1308.3 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Blk
Scientific Background: PDGFRA is one of several protein kinases that can phosphorylate KinSub1DDNYV. Human PDGFRA is a protein-tyrosine kinase of 1089 amino acid length, with a predicted molecular mass of 122,670 Da. It is a member of the TK group of protein kinases in the PDGFR family. This kinase is highly expressed and widely distributed in most tested human tissues. Orthologues are highly conserved in vertebrates and insects. PDGFRA is activated by binding platelet-derived growth factor (PDGF), which induces dimerization and autophosphorylation. Protein interactions are induced with phosphorylation of Y572 and Y574 (with Src), Y720 and Y754 (with SHP2), Y731 and Y742 (with PIK3R1), Y762 (with CDK6 & CrkL), and Y988 and Y1018 (with PLCg1). Aberrant expression of PDGFRA has been linked to developmental abnormalities and has been implicated in multiple disease states in humans. Widespread expression of PDGFRa occurs in renal cell types involved in fibrotic and sclerosing processes (1). PDGFRA induces fibrosis in the repair phase of inflammatory bowel disease and may be involved in the active inflammatory phase (2). PDGFRA has also been linked with the development of atypical chronic myelogenous leukemia, glioblastoma multiforme (GM), melanomas, lung neuroendocrine carcinomas and gastrointestinal stromal cancers.
References
[1] Floege J, Hudkins KL, Davis CL, Schwartz SM, Alpers CE. Expression of PDGF alpha-receptor in renal arteriosclerosis and rejecting renal transplants. J Am Soc Nephrol. 1998 Feb;9(2):211-23. PMID: 9527397
[2] Kumagai S, Ohtani H, Nagai T, Funa K, Hiwatashi NO, Shimosegawa, Nagura H. Tohoku. Platelet-derived growth factor and its receptors are expressed in areas of both active inflammation and active fibrosis in inflammatory bowel disease. J Exp Med. 2001 Sep;195(1):21-33. PMID:11780721
[1] Floege J, Hudkins KL, Davis CL, Schwartz SM, Alpers CE. Expression of PDGF alpha-receptor in renal arteriosclerosis and rejecting renal transplants. J Am Soc Nephrol. 1998 Feb;9(2):211-23. PMID: 9527397
[2] Kumagai S, Ohtani H, Nagai T, Funa K, Hiwatashi NO, Shimosegawa, Nagura H. Tohoku. Platelet-derived growth factor and its receptors are expressed in areas of both active inflammation and active fibrosis in inflammatory bowel disease. J Exp Med. 2001 Sep;195(1):21-33. PMID:11780721
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