Product Name: KinSub1PPLSP
Product Number: PE-01AHF95
Size: 200 µg      Price:99.00
      $US
Peptide Name: KinSub1PPLSP

Product Use: For assaying the phosphotransferase activity of Extracellular regulated protein-serine kinase 2 (p42 MAP kinase, ERK2, UniProt ID P28482). The KinSub1PPLSP peptide demonstrated moderate phosphotransferase activity with ERK2, and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: KinSub1PPLSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.

Peptide Sequence: GGPSPPLSPTPGGYG

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 1339.5 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: ERK2

Scientific Background: ERK2 is one of several protein kinases that can phosphorylate KinSub1PPLSP. Human ERK2 is a protein-serine/threonine kinase of 360 amino acid length, with a predicted molecular mass of 41,390 Da. It is a member of the CMGC group of protein kinases in the MAPK family, and ERK subfamily. This kinase is highly expressed in most tested human tissues. Orthologues of ERK2 appear to be highly conserved in animals, plants and unicellular eukaryotes. Phosphorylation of Thr-185 and Tyr-187 in ERK2 by either MEK1 or MEK2 increases its phosphotransferase activity.