Product Name: KinSub1REGSV
Product Number: PE-01AHM95
Size: 200 µg      Price:99.00
      $US
Peptide Name: KinSub1REGSV

Product Use: For assaying the phosphotransferase activity of Dual specificity protein kinase CLK2 (UniProt ID P49760). The KinSub1REGSV peptide demonstrated medium phosphotransferase activity with CLK2, and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: KinSub1REGSV was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.

Peptide Sequence: GGLGREGSVGVGGHW

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 1423.5 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: CLK2

Scientific Background: CLK2 is one of several protein kinases that can phosphorylate KinSub1REGSV. Human CLK2 is a protein-serine/threonine kinase of 499 amino acid length, with a predicted molecular mass of 60090 Da. It is a member of the CMGC group of protein kinases in the CLK family. This kinase is highly expressed and widely distributed in most tested human tissues. Orthologues of CLK2 are amongst the most highly conserved protein kinases in animals, plants, fungi and unicellular eukaryotes. CLK2 has been shown to phosphorylate SR proteins and to influence alternative splicing in overexpression systems (1). Recent findings demonstrated that the CLK kinases activate PTP-1B family members, and this phosphatase may be an important cellular target for CLK action. Mutations in the CLK2 proteins affect organismal features such as development, behavior, reproduction, and aging as well as cellular features such as the cell cycle, apoptosis, the DNA replication checkpoint, and telomere length (2).