Product Name: KinSub1RGDYE
Product Number: PE-01AHN95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1RGDYE
Product Use: For assaying the phosphotransferase activity of Zeta-chain (TCR) associated protein-tyrosine kinase, 70 kDa (ZAP70, UniProt ID P43403). The KinSub1RGDYE peptide demonstrated very high phosphotransferase activity with Blk, and exhibited medium specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RGDYE was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRERGDYECVGRGG
Product Use: For assaying the phosphotransferase activity of Zeta-chain (TCR) associated protein-tyrosine kinase, 70 kDa (ZAP70, UniProt ID P43403). The KinSub1RGDYE peptide demonstrated very high phosphotransferase activity with Blk, and exhibited medium specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RGDYE was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRERGDYECVGRGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1566.7 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Blk
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1566.7 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Blk
Scientific Background: ZAP70 (SRK) is one of several protein kinases that can phosphorylate KinSub1RGDYE. Human ZAP70 is a protein-tyrosine kinase of 619 amino acid length, with a predicted molecular mass of 69,872 Da. It is a member of the TK group of protein kinases in the Syk family. It is highly expressed in heart, lymph nodes, pancreas, parathyroid gland, spleen, stomach, thymus and tonsils. Orthologues are highly conserved in vertebrates, including amphibians. ZAP70 is activated by phosphorylation at Y319, Y474 and Y493, and inhibited by phosphorylation at Y492, which functions as a docking site for Cbl. Phosphorylation of Y319 also increases PLC-g1+ and Ras activation, and it is an Lck binding site. Phosphorylation of Y315 of ZAP70 induces association with CrkII. Phosphorylation of S520 primes ZAP70 binding to the plasma membrane. ZAP70 mediates T-cell antigen receptor (TCR) signalling. Ligation of the TCR/CD3 receptor in Jurkat T-cells induces complexes which contain ZAP70 (1). TCR zeta chains are initially phosphorylated by Lck, which leads to the recruitment of ZAP70 via its SH2 domain. ZAP70 in turn phosphorylates the zeta-chain dimer in the TCR/CD3-phosphoprotein complex (2). ZAP70 has been linked with severe combined immunodeficiency, chronic arthritis, B-cell chronic lymphocytic leukemia, selective T-cell defects, and neck squamous cell carcinomas.
References
[1] Duplay P, Thome M, Hervé F, Acuto O. p56lck interacts via its src homology 2 domain with the ZAP-70 kinase. J Exp Med. 1994 Apr 1;179(4):1163-72. PMID:8145035 PMCID: PMC2191435
[2] Chan AC, van Oers NS, Tran A, Turka L, Law CL, Ryan JC, Clark EA, Weiss A. Differential expression of ZAP-70 and Syk protein tyrosine kinases, and the role of this family of protein tyrosine kinases in TCR signaling. J Immunol. 1994 May 15;152(10):4758-66. PMID: 8176201
[1] Duplay P, Thome M, Hervé F, Acuto O. p56lck interacts via its src homology 2 domain with the ZAP-70 kinase. J Exp Med. 1994 Apr 1;179(4):1163-72. PMID:8145035 PMCID: PMC2191435
[2] Chan AC, van Oers NS, Tran A, Turka L, Law CL, Ryan JC, Clark EA, Weiss A. Differential expression of ZAP-70 and Syk protein tyrosine kinases, and the role of this family of protein tyrosine kinases in TCR signaling. J Immunol. 1994 May 15;152(10):4758-66. PMID: 8176201
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