Product Name: KinSub1RPRSP
Product Number: PE-01AHV95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1RPRSP
Product Use: For assaying the phosphotransferase activity of calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2, UniProt ID Q96RR4). The KinSub1RPRSP peptide demonstrated moderate phosphotransferase activity with CaMKK2, and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RPRSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRGRPRSPCGGGSG
Product Use: For assaying the phosphotransferase activity of calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2, UniProt ID Q96RR4). The KinSub1RPRSP peptide demonstrated moderate phosphotransferase activity with CaMKK2, and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RPRSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRGRPRSPCGGGSG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1356.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CaMKK2
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1356.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CaMKK2
Scientific Background: CaMKK2 is one of several protein kinases that can phosphorylate KinSub1RPRSP. Human CaMKK2 is a protein-serine/threonine kinase of 588 amino acid length, with a predicted molecular mass of 64,732 Da. It is a member of the Other group of protein kinases in the CAMKK family, and Meta subfamily. This kinase is highly expressed and widely distributed in most tested human tissues. It is broadly distributed in tissues with highest levels in brain, thymus, spleen, and testis (1). Orthologues of CaMKK2 are amongst the most highly conserved protein kinases in animals, plants, fungi and unicellular eukaryotes. CaMKK2 is activated by Ca2+/calmodulin and inhibited following phosphorylation by cAMP-dependent protein kinase (PKA). CaMKK2 undergoes intramolecular autophosphorylation, is regulated by Ca2+/calmodulin and phosphorylates CaMK1 and CaMK4 on Thr-177 and Thr-200, respectively. CaMKK2 also phosphorylates and regulates the activity of AMPK, which is an important regulator of cellular metabolism in response to metabolic stress (2). CaMKK2 has been linked with the development of colorectal adenocarcinomas and lung neuroendocrine carcinomas.
References
[1] Anderson KA, Means RL, Huang QH, Kemp BE, Goldstein EG, Selbert MA, Edelman AM, Fremeau RT, Means AR. Components of a calmodulin-dependent protein kinase cascade. Molecular cloning, functional characterization and cellular localization of Ca2+/calmodulin-dependent protein kinase kinase beta. J Biol Chem. 1998 Nov 27;273(48):31880-9. PMID: 9822657.
[2] Hurley RL, Anderson KA, Franzone JM, Kemp BE, Means AR, Witters LA. The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases. J Biol Chem. 2005 Aug 12;280(32):29060-6. PMID: 15980064.
[1] Anderson KA, Means RL, Huang QH, Kemp BE, Goldstein EG, Selbert MA, Edelman AM, Fremeau RT, Means AR. Components of a calmodulin-dependent protein kinase cascade. Molecular cloning, functional characterization and cellular localization of Ca2+/calmodulin-dependent protein kinase kinase beta. J Biol Chem. 1998 Nov 27;273(48):31880-9. PMID: 9822657.
[2] Hurley RL, Anderson KA, Franzone JM, Kemp BE, Means AR, Witters LA. The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases. J Biol Chem. 2005 Aug 12;280(32):29060-6. PMID: 15980064.
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