Product Name: KinSub1RRDSF
Product Number: PE-01AHX95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1RRDSF
Product Use: For assaying the phosphotransferase activity of MAP kinase-activated protein kinase 3 (MAPKAPK3, UniProt ID Q16644). The KinSub1RRDSF peptide demonstrated high phosphotransferase activity with PAK5 (PAK7), and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RRDSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGLGRRDSFANLGHY
Product Use: For assaying the phosphotransferase activity of MAP kinase-activated protein kinase 3 (MAPKAPK3, UniProt ID Q16644). The KinSub1RRDSF peptide demonstrated high phosphotransferase activity with PAK5 (PAK7), and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RRDSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGLGRRDSFANLGHY
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1618.8 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: PAK5 (PAK7)
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1618.8 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: PAK5 (PAK7)
Scientific Background: MAPKAPK3 is one of several protein kinases that can phosphorylate KinSub1RRDSF. Human MAPKAPK3 is a protein-serine/threonine kinase of 382 amino acid length, with a predicted molecular mass of 42,987 Da. It is a member of the CAMK group of protein kinases in the MAPKAPK family, and MAPKAPK subfamily. This kinase is highly expressed and widely distributed in most tested human tissues. Orthologues of MAPKAPK3 are highly conserved in vertebrates and insects. MAPKAPK3 interacts with TCF3 and with polycomb proteins, such as PCH2 and BMI1/PCGF4. MAPKAPK3 is closely related to MAPKAPK2 sharing 72% nucleotide and 75% amino acid identity (1). MAPKAPK3 is activated by growth inducers and stress stimulation of cells. In vitro studies have demonstrated that ERK1/2, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which indicated the role of MAPKAPK3 as an integrative element of signalling in both mitogen and stress responses (2). MAPKAPK3 has been linked with the development of glioblastoma multiforme (GM).
References
[1] Sithanandam G, Latif F, Duh FM, Bernal R, Smola U, Li H, Kuzmin I, Wixler V, Geil L, Shrestha S. 3pK, a new mitogen-activated protein kinase-activated protein kinase located in the small cell lung cancer tumor suppressor gene region. Mol Cell Biol. 1996 Mar;16(3):868-76. PMID: 8622688 PMCID: PMC231067
[2] Ludwig S, Engel K, Hoffmeyer A, Sithanandam G, Neufeld B, Palm D, Gaestel M, Rapp UR. 3pK, a novel mitogen-activated protein (MAP) kinase-activated protein kinase, is targeted by three MAP kinase pathways. Mol Cell Biol. 1996 Dec;16(12):6687-97. PMID: 8943323 PMCID: PMC231671
[1] Sithanandam G, Latif F, Duh FM, Bernal R, Smola U, Li H, Kuzmin I, Wixler V, Geil L, Shrestha S. 3pK, a new mitogen-activated protein kinase-activated protein kinase located in the small cell lung cancer tumor suppressor gene region. Mol Cell Biol. 1996 Mar;16(3):868-76. PMID: 8622688 PMCID: PMC231067
[2] Ludwig S, Engel K, Hoffmeyer A, Sithanandam G, Neufeld B, Palm D, Gaestel M, Rapp UR. 3pK, a novel mitogen-activated protein (MAP) kinase-activated protein kinase, is targeted by three MAP kinase pathways. Mol Cell Biol. 1996 Dec;16(12):6687-97. PMID: 8943323 PMCID: PMC231671
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