Product Name: KinSub1RRKSV
Product Number: PE-01AJH95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1RRKSV
Product Use: For assaying the phosphotransferase activity of calcium/calmodulin-dependent protein-serine kinase 2 beta (CaMK2b, UniProt ID Q13554). The KinSub1RRKSV peptide demonstrated high phosphotransferase activity with CaMK2b, and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RRKSV was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: RGRGRRKSVGGGGGG
Product Use: For assaying the phosphotransferase activity of calcium/calmodulin-dependent protein-serine kinase 2 beta (CaMK2b, UniProt ID Q13554). The KinSub1RRKSV peptide demonstrated high phosphotransferase activity with CaMK2b, and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RRKSV was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: RGRGRRKSVGGGGGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1412.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CaMK2b
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1412.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CaMK2b
Scientific Background: CaMK2b is a protein-serine/threonine kinase of the CAMK group and CAMK2 family. It is a calcium/calmodulin-dependent protein kinase. Autophosphorylation at T287 increases phosphotransferase activity in a Ca(2+)-independent manner. It is an important kinase in the central nervous system and functions as components in long-term potentiation and neurotransmitter release. It is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplamic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2 isoforms, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. CaMK2b participates in the regulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplamic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates to the control of Ca2+ release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.
References
[1] Tombes RM, Krystal GW. Identification of novel human tumor cell-specific CaMK-II variants. Biochim Biophys Acta. 1997 Mar 1;1355(3):281-92. PMID: 9060999.
[2] Thiagarajan TC, Piedras-Renteria ES, Tsien RW. alpha- and betaCaMKII. Inverse regulation by neuronal activity and opposing effects on synaptic strength. Neuron. 2002 Dec 19;36(6):1103-14. PMID: 12495625.
[1] Tombes RM, Krystal GW. Identification of novel human tumor cell-specific CaMK-II variants. Biochim Biophys Acta. 1997 Mar 1;1355(3):281-92. PMID: 9060999.
[2] Thiagarajan TC, Piedras-Renteria ES, Tsien RW. alpha- and betaCaMKII. Inverse regulation by neuronal activity and opposing effects on synaptic strength. Neuron. 2002 Dec 19;36(6):1103-14. PMID: 12495625.
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