Product Name: KinSub1RRLSY
Product Number: PE-01AJK95
Size: 200 µg      Price:99.00
      $US
Peptide Name: KinSub1RRLSY

Product Use: For assaying the phosphotransferase activity of Myotonic dystrophy kinase-related CDC42-binding kinase beta (MRCKb, UniProt ID Q9Y5S2). The KinSub1RRLSY peptide demonstrated medium phosphotransferase activity with CHK2, and exhibited medium specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: KinSub1RRLSY was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.

Peptide Sequence: HIRGRRLSYVGKTGY

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 1762 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: CHK2

Scientific Background: MRCKb is one of several protein kinases that can phosphorylate KinSub1RRLSY. Human MRCKb is a protein-serine/threonine kinase of 1711 amino acid length, with a predicted molecular mass of 194,315 Da. It is a member of the AGC group of protein kinases in the DMPK family, and GEK subfamily (1). This kinase is moderate to highly expressed in most tested human tissues. Orthologues of MRCKb are highly conserved in vertebrates, including amphibians. MRCKb interacts tightly with GTP-bound but not GDP-bound CDC42 and RhoA, and this permits N-terminus-mediated dimerization and activation of the phosphotransferase activity of MRCKb by transautophosphorylation. MRCKb mediates RhoA and Cdc42 regulation of actin cytoskeletal reorganization and neurite outgrowth (2). Effects of the repeat expansion on the MRCKb gene may be responsible for muscle and heart features of Myotonic Dystrophy. MRCKb has also been linked with the development of breast (ductal), colorectal and lung large cell carcinomas.