Product Name: KinSub1RRLSY
Product Number: PE-01AJK95
Size: | 200 µg | | Price: | 99.00 |
| | | $US | |
Peptide Name: KinSub1RRLSY
Product Use: For assaying the phosphotransferase activity of Myotonic dystrophy kinase-related CDC42-binding kinase beta (MRCKb, UniProt ID Q9Y5S2). The KinSub1RRLSY peptide demonstrated medium phosphotransferase activity with CHK2, and exhibited medium specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RRLSY was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: HIRGRRLSYVGKTGY
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1762 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CHK2
Scientific Background: MRCKb is one of several protein kinases that can phosphorylate KinSub1RRLSY. Human MRCKb is a protein-serine/threonine kinase of 1711 amino acid length, with a predicted molecular mass of 194,315 Da. It is a member of the AGC group of protein kinases in the DMPK family, and GEK subfamily (1). This kinase is moderate to highly expressed in most tested human tissues. Orthologues of MRCKb are highly conserved in vertebrates, including amphibians. MRCKb interacts tightly with GTP-bound but not GDP-bound CDC42 and RhoA, and this permits N-terminus-mediated dimerization and activation of the phosphotransferase activity of MRCKb by transautophosphorylation. MRCKb mediates RhoA and Cdc42 regulation of actin cytoskeletal reorganization and neurite outgrowth (2). Effects of the repeat expansion on the MRCKb gene may be responsible for muscle and heart features of Myotonic Dystrophy. MRCKb has also been linked with the development of breast (ductal), colorectal and lung large cell carcinomas.