Product Name: KinSub1RRMSF
Product Number: PE-01AJL95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1RRMSF
Product Use: For assaying the phosphotransferase activity of Protein kinase C iota type (PKCi, UniProt ID P41743). The KinSub1RRMSF peptide demonstrated very high phosphotransferase activity with PKCi, and exhibited medium specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RRMSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRGRRMSFGVGGGG
Product Use: For assaying the phosphotransferase activity of Protein kinase C iota type (PKCi, UniProt ID P41743). The KinSub1RRMSF peptide demonstrated very high phosphotransferase activity with PKCi, and exhibited medium specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RRMSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRGRRMSFGVGGGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1406.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: PKCi
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1406.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: PKCi
Scientific Background: PKCi is one of several protein kinases that can phosphorylate KinSub1RRMSF. Human PKCi is a protein-serine/threonine kinase of 596 amino acid length, with a predicted molecular mass of 68,262 Da. It is a member of the AGC group of protein kinases in the PKC family, and Iota subfamily. This kinase is highly expressed and widely distributed in most tested human tissues. Orthologues of PKCi are highly conserved in vertebrates and insects. Phosphorylation of Y325, T403 and possible T412 of PKCi increases phosphotransferase activity. PKCi has been linked with the development of melanomas (metastatic). The amino acid sequence of PKCi showed greatest homology to PKCzeta, with 72% identity overall rising to 84% in the catalytic domain. PKCi has been implicated in Ras signalling and is a critical downstream effector of oncogenic Ras in the colonic epithelium. Transgenic mice expressing constitutively active PKCi in the colon are highly susceptible to carcinogen-induced colon carcinogenesis (1).
© Kinexus Bioinformatics Corporation 2017