Product Name: KinSub1RTGSG
Product Number: PE-01AJN95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1RTGSG
Product Use: For assaying the phosphotransferase activity of MAP/microtubule affinity-regulating protein-serine kinase 1 (MARK1, UniProt ID Q9P0L2). The KinSub1RTGSG peptide demonstrated high phosphotransferase activity with TXK, and exhibited low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RTGSG was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: KGLRRTGSGCGGGHG
Product Use: For assaying the phosphotransferase activity of MAP/microtubule affinity-regulating protein-serine kinase 1 (MARK1, UniProt ID Q9P0L2). The KinSub1RTGSG peptide demonstrated high phosphotransferase activity with TXK, and exhibited low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RTGSG was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: KGLRRTGSGCGGGHG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1398.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: TXK
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1398.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: TXK
Scientific Background: MARK1 is one of several protein kinases that can phosphorylate KinSub1RTGSG. Human MARK1 is a protein-serine/threonine kinase of 795 amino acid length, with a predicted molecular mass of 89,003 Da. It is a member of the CAMK group of protein kinases in the CAMKL family, and MARK subfamily. This kinase is highly expressed and widely distributed in most tested human tissues except apparently in the brain and spinal cord. Orthologues of MARK1 are highly conserved in vertebrates and insects. MARK1 is activated by phosphorylation on T215 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. MARK1 phosphorylates microtubule-associated proteins and trigger microtubule disruption (1). Gene mutation studies performed in mice revealed that after targeted disruption of the MARK1 gene, the mice lacked the ability to drink, and displayed hind leg motor dysfunction (2). MARK1 has been linked with the development of gastric adenocarcinomas and ovarian serous carcinomas.
References
[1] Drewes G, Ebneth A, Preuss U, Mandelkow EM, Mandelkow E. MARK, a novel family of protein kinases that phosphorylate microtubule-associated proteins and trigger microtubule disruption. Cell. 1997 Apr 18;89(2):297-308. PMID: 910848
[2] Nakayama M, Iida M, Koseki H, Ohara O. A gene-targeting approach for functional characterization of KIAA genes encoding extremely large proteins. FASEB J. 2006 Aug;20(10):1718-20. Epub 2006 Jun 28. PMID: 16807365
[1] Drewes G, Ebneth A, Preuss U, Mandelkow EM, Mandelkow E. MARK, a novel family of protein kinases that phosphorylate microtubule-associated proteins and trigger microtubule disruption. Cell. 1997 Apr 18;89(2):297-308. PMID: 910848
[2] Nakayama M, Iida M, Koseki H, Ohara O. A gene-targeting approach for functional characterization of KIAA genes encoding extremely large proteins. FASEB J. 2006 Aug;20(10):1718-20. Epub 2006 Jun 28. PMID: 16807365
© Kinexus Bioinformatics Corporation 2017