Product Name: KinSub2DDDYG
Product Number: PE-01AJS95
Size: 200 µg      Price:99.00
      $US
Peptide Name: KinSub2DDDYG

Product Use: For assaying the phosphotransferase activity of Macrophage-stimulating protein receptor alpha chain (Ron, UniProt ID Q04912). The KinSub2DDDYG peptide demonstrated very high phosphotransferase activity with Blk, and exhibited medium specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: KinSub2DDDYG was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.

Peptide Sequence: GGGEDDDYGGFGGGG

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 1315.2 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: Blk

Scientific Background: RON is one of several protein kinases that can phosphorylate KinSub2DDDYG. Human RON is a protein-tyrosine kinase of 1400 amino acid length, with a predicted molecular mass of 152,271 Da. It is a member of the TK group of protein kinases in the Met family. This kinase is highly expressed and widely distributed in most tested human tissues. Orthologues of RON are highly conserved in vertebrates and insects. RON is activated by binding macrophage stimulating factor (MSF) and related to Met (1). Phosphorylation Y1198 and Y1288 increases phosphotransferase activity. Phosphorylation at S1394 induces interaction with 14-3-3 beta. RON interacts with the HYAL2 receptor protein, rendering it functionally inactive. HYAL2 is a candidate tumour-suppressor glycosylphosphatidylinositol-anchored cell-surface protein that serves as an entry receptor for jaagsiekte retrovirus, a virus that causes contagious lung cancer in sheep that is morphologically similar to human bronchioloalveolar carcinoma. It was shown that RON liberated from the association with HYAL2 becomes functionally active and activates the Akt and mitogen-activated protein kinase pathways (2). RON has also been linked with the development of breast, head and neck, ovarian, renal and breast cancers, and pulmonary adenomas.
References

[1] Wang MH, Ronsin C, Gesnel MC, Coupey L, Skeel A, Leonard EJ, Breathnach R. Identification of the RON gene product as the receptor for the human macrophage stimulating protein. Science. 1994 Oct 7;266(5182):117-9. PMID: 7939629

[2] Li BQ, Wang MH, Kung HF, Ronsin C, Breathnach R, Leonard EJ, Kamata T. Macrophage-stimulating protein activates Ras by both activation and translocation of SOS nucleotide exchange factor. Biochem Biophys Res Commun. 1995 Nov 2;216(1):110-8. PMID: 7488076