Product Name: KinSub2MTMTP
Product Number: PE-01AJY95
Size: 200 µg      Price:99.00
      $US
Peptide Name: KinSub2MTMTP

Product Use: For assaying the phosphotransferase activity of Fibroblast growth factor receptor-tyrosine kinase 1; Basic fibroblast growth factor receptor 1 (FGFR1, UniProt ID P11362). The KinSub2MTMTP peptide demonstrated medium phosphotransferase activity with Src, and exhibited very low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: KinSub2MTMTP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.

Peptide Sequence: GGRSMTMTPYVGTYG

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 1576.8 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: Src

Scientific Background: FGFR1 is one of several protein kinases that can phosphorylate KinSub2MTMTP. Human FGFR1 (also known as FLT2) is a receptor protein-tyrosine kinase of 822 amino acid length, with a predicted molecular mass of 91,868 Da. It is a member of the TK group of protein kinases in the Fibroblast Growth Factor Receptor family, which includes 4 high-affinity receptors for FGF's 1 to 17. It is highly expressed and widely distributed in most tested human tissues. Orthologues are highly conserved in vertebrates, including amphibians. FGF Receptors plays an important role in multiple biological processes, including mesoderm induction and patterning, cell growth and migration, organ formation and bone growth (1). FGFR1 is activated by basic-FGF, which induces dimerization and transautophosphorylation. Phosphorylation of Y653 and Y654 increases kinase activity. Phosphorylation of Y766 induces interaction with PLCg1. Phosphorylation of Y154 induces receptor internalization. FGFR1 is alternatively spliced generating multiple variants that are differentially expressed during embryogenesis and in the adult body (2). FGFR1 has been linked with the development of Pfeiffer Syndrome (PS), Kallmann Syndrome Type 2 (KAL2), Stem Cell Leukemia Lymphoma Syndrome (SCLL), breast carcinomas (ductal), lung bronchoalveolar carcinomas and lung large cell carcinomas.
References

[1] Xu X, Weinstein M, Li C, Deng C. Fibroblast growth factor receptors (FGFRs) and their roles in limb development. Cell Tissue Res. 1999 Apr;296(1):33-43. PMID: 10199963

[2] Groth C, Lardelli M. The structure and function of vertebrate fibroblast growth factor receptor 1. Int J Dev Biol. 2002;46(4):393-400. PMID:12141425