Product Name: KinSub2RRDSF
Product Number: PE-01AKA95
Size: 200 µg      Price:99.00
      $US
Peptide Name: KinSub2RRDSF

Product Use: For assaying the phosphotransferase activity of Ribosomal S6 protein-serine kinase 1; Ribosomal protein S6 kinase alpha 2 (RSK1, UniProt ID Q15418). The KinSub2RRDSF peptide demonstrated high phosphotransferase activity with Brk, and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: KinSub2RRDSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.

Peptide Sequence: GGRGRRDSFCGGGYG

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 1500.6 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: Brk

Scientific Background: RSK1 is one of several protein kinases that can phosphorylate KinSub2RRDSF. Human RSK1 is a protein-serine/threonine kinase of 735 amino acid length, with a predicted molecular mass of 82,723 Da. It is a member of the AGC group of protein kinases in the RSK family, and RSK subfamily. This kinase is highly expressed and widely distributed in most tested human tissues. RSK1 transcript is present in lymphocytes, skeletal muscle, liver, and adipose tissue (1). Orthologues of RSK1 are highly conserved in mammals and birds. RSK1 containing two distinct kinase catalytic domains. RSK1 is activated by phosphorylation at S363, S380 and T573. Phosphorylation at S221 by PDK1 is required for increased phosphotransferase activity, but is insufficient by itself. Phosphorylation at S154 induces interaction with 14-3-3 and inhibits catalytic activity. RSKs are implicated in the activation of the mitogen-activated kinase (MAPK) cascade and the stimulation of cell proliferation and differentiation (2). RSK1 forms a complex with either ERK1 or ERK2 in quiescent cells, and phosphorylation of S732 in response to mitogenic stimulation of cells permits dissociation of RSK-ERK complexes.
References

[1] Moller DE, Xia CH, Tang W, Zhu AX, Jakubowski M. Human rsk isoforms: cloning and characterization of tissue-specific expression. Am J Physiol. 1994 Feb;266(2 Pt 1):C351-9. PMID:8141249

[2] Gross SD, Schwab MS, Lewellyn AL, Maller JL. Induction of metaphase arrest in cleaving Xenopus embryos by the protein kinase p90RSK. Science. 1999 Nov 12;286(5443):1365-7. PMID:10558992