Product Name: KinSub2RRGSF
Product Number: PE-01AKC95
Size: 200 µg      Price:99.00
      $US
Peptide Name: KinSub2RRGSF

Product Use: For assaying the phosphotransferase activity of Protein-serine kinase C delta (PKCd, UniProt ID Q05655). The KinSub2RRGSF peptide demonstrated high phosphotransferase activity with PKCg, and exhibited low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: KinSub2RRGSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.

Peptide Sequence: GGLGRRGSFCNGGHY

Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 1536.7 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: PKCg

Scientific Background: PKCd is one of several protein kinases that can phosphorylate KinSub2RRGSF. Human PKCd is a protein-serine/threonine kinase of 676 amino acid length, with a predicted molecular mass of 77,505 Da. It is a member of the AGC group of protein kinases in the PKC family, and Delta subfamily. This kinase is highly expressed and widely distributed in most tested human tissues, and is a major isoform of PKC expressed in hemopoietic cells (1). Orthologues of PKCd are highly conserved in vertebrates and insects. PKCd is activated by phosphorylation at Y313, Y514, Y525, and S645. Phosphorylation of T507 increases phosphotransferase activity and induces degradation of PKC-delta. Phosphorylation of Y52, Y64, Y187, and Y567 inhibits phosphotransferase activity. PKCd has been linked with the development of colon cancer and cancer. PKCd shows strict dependence on the presence of phospholipids, but displays no activation by Ca2+ (2). Phosphatidylinositol is the most potent activator of PKCd. PKC delta is involved in fundamental cellular functions regulated by diacylglycerols, which is mimicked by phorbol esters.
References

[1] Mischak H, Bodenteich A, Kolch W, Goodnight J, Hofer F, Mushinski JF. Mouse protein kinase C-delta, the major isoform expressed in mouse hemopoietic cells: sequence of the cDNA, expression patterns, and characterization of the protein. Biochemistry. 1991 Aug 13;30(32):7925-31. PMID: 1868068

[2] Leibersperger H, Gschwendt M, Gernold M, Marks F. Immunological demonstration of a calcium-unresponsive protein kinase C of the delta-type in different species and murine tissues. Predominance in epidermis. J Biol Chem. 1991 Aug 5;266(22):14778-84. PMID: 1860875