Product Name: KinSub2RRMSF
Product Number: PE-01AKG95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub2RRMSF
Product Use: For assaying the phosphotransferase activity of Mitogen & stress-activated protein-serine kinase 1; Ribosomal protein S6 kinase alpha 5 (MSK1, UniProt ID O75582). The KinSub2RRMSF peptide demonstrated high phosphotransferase activity with Pim1, and exhibited very low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub2RRMSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRGRRMSFGFGGHY
Product Use: For assaying the phosphotransferase activity of Mitogen & stress-activated protein-serine kinase 1; Ribosomal protein S6 kinase alpha 5 (MSK1, UniProt ID O75582). The KinSub2RRMSF peptide demonstrated high phosphotransferase activity with Pim1, and exhibited very low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub2RRMSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRGRRMSFGFGGHY
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1640.9 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Pim1
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1640.9 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Pim1
Scientific Background: MSK1 is one of several protein kinases that can phosphorylate KinSub2RRMSF. Human Msk1 is a protein-serine/threonine kinase of 802 amino acid length, with a predicted molecular mass of 89,865 Da. It is a member of the AGC group of protein kinases in the RSK family, and Msk subfamily. This kinase is highly expressed and widely distributed in most tested human tissues, including highest levels in brain, muscle, and placenta. Orthologues of MSK1 are highly conserved in vertebrates and insects. MSK1 features two protein kinase domains and shares 43% protein sequence identity with the MAPKAPK1 (RSK) isoforms. MSK1 is activated by phosphorylation at S212, S360, S376, T581 and T700. It forms a complex with either ERK1 or ERK2 in quiescent cells, which transiently dissociates following mitogenic stimulation. Immunoelectron microscopy localized MSK1 to the nucleus. MSK1 is activated in vitro and in vivo by either ERK1/2 or JNK proteins (1). MSK1 rather than MAPKAPK1 or MAPKAP-K2/K3, mediates activation of the cAMP response element-binding protein and activating transcription factor-1 by either growth factors or stress signals.
© Kinexus Bioinformatics Corporation 2017