Product Name: KinSub3DDDYV
Product Number: PE-01AKK95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub3DDDYV
Product Use: For assaying the phosphotransferase activity of Activin receptor type-2B (ACTR2B, UniProt ID Q13705). The KinSub3DDDYV peptide demonstrated high phosphotransferase activity with Brk, and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub3DDDYV was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGGEDDDYVCPGGGG
Peptide Modifications N Terminus: Free amino
Product Use: For assaying the phosphotransferase activity of Activin receptor type-2B (ACTR2B, UniProt ID Q13705). The KinSub3DDDYV peptide demonstrated high phosphotransferase activity with Brk, and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub3DDDYV was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGGEDDDYVCPGGGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1353.3 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Brk
Peptide Molecular Mass Calculated: 1353.3 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Brk
Scientific Background: ACTR2B is one of several protein kinases that can phosphorylate KinSub3DDDYV. Human ACTR2B is a protein-serine/threonine kinase of 512 amino acid length, with a predicted molecular mass of 57,724 Da. It is a member of the TKL group of protein kinases in the STKR family, and Type2 subfamily. This kinase is moderate to highly expressed in most tested human tissues. Orthologues of ACTR2B appear to be highly restricted in their distribution in non-simians. ACTR2B is activated by binding activin and is also known as the Activin A receptor, type IIB. Activins belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related proteins that signal through a heteromeric complex of receptor-serine kinases, which include at least two type I (I and IB) and two type II (II and IIB) receptors (1). ACVR2B features a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted protein-serine/threonine kinase specificity. ACVR2B is help to regulate muscle growth (2). ACTR2B has been linked with the development of left-right axis malformations and visceral heterotaxy autosomal type 4 (htX4).
References
[1] Bondestam J, Horelli-Kuitunen N, Hildén K, Ritvos O, Aaltonen Assignment of ACVR2 and ACVR2B the human activin receptor type II and IIB genes to chromosome bands 2q22.2-->q23.3 and 3p22 and the human follistatin gene (FST) to chromosome 5q11.2 by FISH. J. Cytogenet Cell Genet. 1999;87(3-4):219-20. PMID: 10702675.
[2] Lee SJ, Reed LA, Davies MV, Girgenrath S, Goad ME, Tomkinson KN, Wright JF, Barker C, Ehrmantraut G, Holmstrom J, Trowell B, Gertz B, Jiang MS, Sebald SM, Matzuk M, Li E, Liang LF, Quattlebaum E, Stotish RL, Wolfman NM. Regulation of muscle growth by multiple ligands signaling through activin type II receptors. Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):18117-22. PMID: 16330774.
[1] Bondestam J, Horelli-Kuitunen N, Hildén K, Ritvos O, Aaltonen Assignment of ACVR2 and ACVR2B the human activin receptor type II and IIB genes to chromosome bands 2q22.2-->q23.3 and 3p22 and the human follistatin gene (FST) to chromosome 5q11.2 by FISH. J. Cytogenet Cell Genet. 1999;87(3-4):219-20. PMID: 10702675.
[2] Lee SJ, Reed LA, Davies MV, Girgenrath S, Goad ME, Tomkinson KN, Wright JF, Barker C, Ehrmantraut G, Holmstrom J, Trowell B, Gertz B, Jiang MS, Sebald SM, Matzuk M, Li E, Liang LF, Quattlebaum E, Stotish RL, Wolfman NM. Regulation of muscle growth by multiple ligands signaling through activin type II receptors. Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):18117-22. PMID: 16330774.
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