Product Name: KinSub4RPLSP
Product Number: PE-01AKT95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub4RPLSP
Product Use: For assaying the phosphotransferase activity of Mitogen-activated protein-serine kinase p38 beta; Mitogen-activated protein kinase 11 (p38b, UniProt ID Q15759). The KinSub4RPLSP peptide demonstrated medium phosphotransferase activity with Brk, and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub4RPLSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GIRSRPLSPCPGTYG
Product Use: For assaying the phosphotransferase activity of Mitogen-activated protein-serine kinase p38 beta; Mitogen-activated protein kinase 11 (p38b, UniProt ID Q15759). The KinSub4RPLSP peptide demonstrated medium phosphotransferase activity with Brk, and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub4RPLSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GIRSRPLSPCPGTYG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1559.8 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Brk
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1559.8 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Brk
Scientific Background: p38b is one of several protein kinases that can phosphorylate KinSub4RPLSP. Human p38b (also known as MAPK11) is a protein-serine/threonine kinase of 364 amino acid length, with a predicted molecular mass of 41357 Da. It is a member of the CMGC group of protein kinases in the MAPK family, and p38 subfamily. This kinase is moderate to highly expressed in most tested human tissues. Orthologues of p38b are highly conserved in animals and plants. p38b is activated by both proinflammatory cytokines and environmental stress (1). p38b is directly activated by phosphorylation at Y182 and probably T180 by MEK6 (MAP2K6, MKK6). Transcription factor ATF2/CREB2 has been shown to be a substrate of this kinase (2). Alternatively spliced transcript variants encoding the same protein have been observed. p38b has been linked with the development of inflammation and autoimmunity, diabetes and lung neuroendocrine carcinomas.
References
[1] Jiang Y, Chen C, Li Z, Guo W, Gegner JA, Lin S, Han J. Characterization of the structure and function of a new mitogen-activated protein kinase (p38beta). J Biol Chem. 1996 Jul 26;271(30):17920-6. PMID:8663524
[2] Stein B, Yang MX, Young DB, Janknecht R, Hunter T, Murray BW, Barbosa MS. p38-2, a novel mitogen-activated protein kinase with distinct properties. J Biol Chem. 1997 Aug 1;272(31):19509-17. PMID: 9235954
[1] Jiang Y, Chen C, Li Z, Guo W, Gegner JA, Lin S, Han J. Characterization of the structure and function of a new mitogen-activated protein kinase (p38beta). J Biol Chem. 1996 Jul 26;271(30):17920-6. PMID:8663524
[2] Stein B, Yang MX, Young DB, Janknecht R, Hunter T, Murray BW, Barbosa MS. p38-2, a novel mitogen-activated protein kinase with distinct properties. J Biol Chem. 1997 Aug 1;272(31):19509-17. PMID: 9235954
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