Product Name: KinSub4RRGSF
Product Number: PE-01AKV95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub4RRGSF
Product Use: For assaying the phosphotransferase activity of Mitogen-activated protein kinase kinase kinase 5 (ASK1, UniProt ID Q99683). The KinSub4RRGSF peptide demonstrated high phosphotransferase activity with PAK5 (PAK7), and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub4RRGSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: HFRGRRGSFGFGPGG
Product Use: For assaying the phosphotransferase activity of Mitogen-activated protein kinase kinase kinase 5 (ASK1, UniProt ID Q99683). The KinSub4RRGSF peptide demonstrated high phosphotransferase activity with PAK5 (PAK7), and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub4RRGSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: HFRGRRGSFGFGPGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1590.8 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: PAK5 (PAK7)
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1590.8 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: PAK5 (PAK7)
Scientific Background: ASK1 is one of several protein kinases that can phosphorylate KinSub4RRGSF. Human ASK1 (also known as MAPKKK5) is a protein-serine/threonine kinase of 1374 amino acid length, with a predicted molecular mass of 154,537 Da. It is a member of the STE group of protein kinases in the STE11 family. This kinase is moderate to highly expressed in most tested human tissues. Orthologues of ASK1 are highly conserved in mammals and birds. Overexpression of ASK1 induces apoptotic cell death. ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the tumour necrosis factor-alpha (TNF) signalling pathway (1). ASK1 is activated by phosphorylation at T838 by MAP3K6 and inhibited by phosphorylation at S966 and S1033. ASK1 phosphorylates and activates MKK3, MKK4 (SEK1), and MKK6. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK (2).
References
[1] Ichijo H, Nishida E, Irie K, ten Dijke P, Saitoh M, Moriguchi T, Takagi M, Matsumoto K, Miyazono K, Gotoh Y. Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science. 1997 Jan 3;275(5296):90-4. PMID: 8974401.
[2] Nishitoh H, Saitoh M, Mochida Y, Takeda K, Nakano H, Rothe M, Miyazono K, Ichijo H. ASK1 is essential for JNK/SAPK activation by TRAF2. Mol Cell. 1998 Sep;2(3):389-95. PMID: 9774977.
[1] Ichijo H, Nishida E, Irie K, ten Dijke P, Saitoh M, Moriguchi T, Takagi M, Matsumoto K, Miyazono K, Gotoh Y. Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science. 1997 Jan 3;275(5296):90-4. PMID: 8974401.
[2] Nishitoh H, Saitoh M, Mochida Y, Takeda K, Nakano H, Rothe M, Miyazono K, Ichijo H. ASK1 is essential for JNK/SAPK activation by TRAF2. Mol Cell. 1998 Sep;2(3):389-95. PMID: 9774977.
© Kinexus Bioinformatics Corporation 2017