Product Name: KinSub4RRKSF
Product Number: PE-01AKW95
Size: 200 µg      Price:99.00
      $US
Peptide Name: KinSub4RRKSF

Product Use: For assaying the phosphotransferase activity of Protein kinase C eta type (PKCh, UniProt ID P24723). The KinSub4RRKSF peptide demonstrated high phosphotransferase activity with TXK, and exhibited low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: KinSub4RRKSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.

Peptide Sequence: GLRSRRKSFCGGMGG

Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 1567.9 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: TXK

Scientific Background: PKCh is one of several protein kinases that can phosphorylate KinSub4RRKSF. Human PKC-eta is a protein-serine/threonine kinase of 683 amino acid length, with a predicted molecular mass of 77,828 Da. It is a member of the AGC group of protein kinases in the PKC family, and Eta subfamily. This kinase is highly expressed and widely distributed in most tested human tissues except in the brain and spinal cord, where it is more moderately expressed. Orthologues of PKCh are highly conserved in vertebrates, including amphibians. Phosphorylation of T513 of PKC-eta by PDK1 induces its preactivation, which permits autophosphorylation at T656 and T675, which are required for full activation. PKCh is predominantly expressed in squamous cell epithelia and induces terminal differentiation of keratinocytes. PKCh that is endogenously expressed or overexpressed is found to associate with the cyclin E/cdk2/p21 complex in keratinocytes of mice and humans (1). PKCh has been linked with the development of renal cell carcinomas and colorectal adenocarcinomas.