Product Name: KinSub5RPLSP
Product Number: PE-01AKZ95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub5RPLSP
Product Use: For assaying the phosphotransferase activity of Cyclin-dependent protein-serine kinase 7 (CDK7, UniProt ID P50613). The KinSub5RPLSP peptide demonstrated moderate phosphotransferase activity with CDK7, and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub5RPLSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRSRPLSPGGGGYG
Product Use: For assaying the phosphotransferase activity of Cyclin-dependent protein-serine kinase 7 (CDK7, UniProt ID P50613). The KinSub5RPLSP peptide demonstrated moderate phosphotransferase activity with CDK7, and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub5RPLSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRSRPLSPGGGGYG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1373.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CDK7
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1373.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CDK7
Scientific Background: CDK7 is one of several protein kinases that can phosphorylate KinSub5RPLSP. Human CDK7 is a protein-serine/threonine kinase of 346 amino acid length, with a predicted molecular mass of 39,038 Da. It is a member of the CMGC group of protein kinases in the CDK family, and CDK7 subfamily. This kinase is moderate to highly expressed in most tested human tissues. Orthologues of CDK7 are amongst the most highly conserved protein kinases in animals, plants, fungi and unicellular eukaryotes. CDK7 is activated by phosphorylation at T170 and is complexed with cyclin H (CCNH) and MAT1 (1). Phosphorylation of S164 by CDK1 during mitosis inactivates CDK7. CDK7 functions as a CDK-activating kinase (CAK) for targets such as CDK1. CDK7 is an essential component of the transcription factor TFIIH that is involved in transcription initiation and DNA repair. CDK7 is believed to serve as a direct link between the regulation of transcription and the cell cycle (2).
References
[1] Fisher RP, Morgan DO. A novel cyclin associates with MO15/CDK7 to form the CDK-activating kinase. Cell. 1994 Aug 26;78(4):713-24. PMID: 8069918.
[2] Larochelle S, Merrick KA, Terret ME, Wohlbold L, Barboza NM, Zhang C, Shokat KM, Jallepalli PV, Fisher RP. Requirements for Cdk7 in the assembly of Cdk1/cyclin B and activation of Cdk2 revealed by chemical genetics in human cells. Mol Cell. 2007 Mar 23;25(6):839-50. PMID: 17386261.
[1] Fisher RP, Morgan DO. A novel cyclin associates with MO15/CDK7 to form the CDK-activating kinase. Cell. 1994 Aug 26;78(4):713-24. PMID: 8069918.
[2] Larochelle S, Merrick KA, Terret ME, Wohlbold L, Barboza NM, Zhang C, Shokat KM, Jallepalli PV, Fisher RP. Requirements for Cdk7 in the assembly of Cdk1/cyclin B and activation of Cdk2 revealed by chemical genetics in human cells. Mol Cell. 2007 Mar 23;25(6):839-50. PMID: 17386261.
© Kinexus Bioinformatics Corporation 2017