Product Name: KinSub5RRKSF
Product Number: PE-01ALC95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub5RRKSF
Product Use: For assaying the phosphotransferase activity of Serine/threonine-protein kinase receptor R3 (ALK1, UniProt ID P37023). The KinSub5RRKSF peptide demonstrated high phosphotransferase activity with ALK1, and exhibited medium specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub5RRKSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRGRRKSFCGGGGG
Product Use: For assaying the phosphotransferase activity of Serine/threonine-protein kinase receptor R3 (ALK1, UniProt ID P37023). The KinSub5RRKSF peptide demonstrated high phosphotransferase activity with ALK1, and exhibited medium specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub5RRKSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRGRRKSFCGGGGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1407.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: ALK1
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1407.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: ALK1
Scientific Background: ALK1 is one of several protein kinases that can phosphorylate KinSub5RRKSF. Human ALK1 is a protein-serine/threonine kinase of 503 amino acid length, with a predicted molecular mass of 56,124 Da. It is a member of the TKL group of protein kinases in the STKR family, and Type1 subfamily. This kinase is highly expressed and widely distributed in most tested human tissues. Orthologues of ALK1 are highly conserved in mammals and birds. ALK1 functions as a TGF-beta type I receptor (1). It is activated by binding activin, which induces heterodimerization and autophosphorylation. Distinct Smad proteins (i.e., Smad2/Smad3 and Smad1/Smad5) show interaction with ALK1 and mediate TGF-beta signaling. Northern blot and RT-PCR analysis show that ALK1 specifically induces expression of Smad6, Smad7, Id1, Id2, endoglin, STAT1, and interleukin 1 receptor in endothelial cells. ALK1 has been linked with the development of Hereditary Hemorrhagic Telangiectasia Type 2 (HHT2). ALK1 expression in inflammatory myofibroblastic tumours of the urinary bladder have also been reported (2).
References
[1] Ota T, Fujii M, Sugizaki T, Ishii M, Miyazawa K, Aburatani H, Miyazono K. Targets of transcriptional regulation by two distinct type I receptors for transforming growth factor-beta in human umbilical vein endothelial cells. J Cell Physiol. 2002 Dec;193(3):299-318. PMID: 12384983.
[2] Tsuzuki T, Magi-Galluzzi C, Epstein JI. ALK-1 expression in inflammatory myofibroblastic tumor of the urinary bladder. Am J Surg Pathol. 2004 Dec;28(12):1609-14. PMID: 15577680.
[1] Ota T, Fujii M, Sugizaki T, Ishii M, Miyazawa K, Aburatani H, Miyazono K. Targets of transcriptional regulation by two distinct type I receptors for transforming growth factor-beta in human umbilical vein endothelial cells. J Cell Physiol. 2002 Dec;193(3):299-318. PMID: 12384983.
[2] Tsuzuki T, Magi-Galluzzi C, Epstein JI. ALK-1 expression in inflammatory myofibroblastic tumor of the urinary bladder. Am J Surg Pathol. 2004 Dec;28(12):1609-14. PMID: 15577680.
© Kinexus Bioinformatics Corporation 2017