Product Name: KinSub7RRGSF
Product Number: PE-01ALJ95
Size: | 200 µg | | Price: | 99.00 |
| | | $US | |
Peptide Name: KinSub7RRGSF
Product Use: For assaying the phosphotransferase activity of NIMA (never-in-mitosis)-related protein-serine kinase 6 (NEK6, UniProt ID Q9HC98 ). The KinSub7RRGSF peptide demonstrated high phosphotransferase activity with PKCi, and exhibited medium specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub7RRGSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GFRSRRGSFRGGGHG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1589.8 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: PKCi
Scientific Background: Nek6 is one of several protein kinases that can phosphorylate KinSub7RRGSF. Human Nek6 is a protein-serine/threonine kinase of 313 amino acid length, with a predicted molecular mass of 35,714 Da. It is a member of the Other group of protein kinases in the Nek family. This kinase is highly expressed and widely distributed in most tested human tissues except apparently in the brain and spinal cord. Orthologues of Nek6 are highly conserved in mammals and birds. Nek6 is closely related in its catalytic domain to the protein-serine/threonine kinase NimA of Aspergillus nidulans (1). Nek6 is phosphorylated and activated during M phase of the cell cycle (2), and its inhibition facilitates apoptosis. Inhibition of Nek6 function by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA arrests cells in M phase and triggers apoptosis.