Product Name: KinSub8RRKSF
Product Number: PE-01ALN95
Size: 200 µg      Price:99.00
      $US
Peptide Name: KinSub8RRKSF

Product Use: For assaying the phosphotransferase activity of Homeodomain-interacting protein kinase 3 (HIPK3, UniProt ID Q9H422). The KinSub8RRKSF peptide demonstrated very high phosphotransferase activity with CHK2, and exhibited low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: KinSub8RRKSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.

Peptide Sequence: GGRKRRKSFRRRGGG

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 1730 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: CHK2

Scientific Background: HIPK3 is one of several protein kinases that can phosphorylate KinSub8RRKSF. Human HIPK3 is a protein-serine/threonine kinase of 1215 amino acid length, with a predicted molecular mass of 133,743 Da. It is a member of the CMGC group of protein kinases in the DYRK family, and HIPK subfamily. This kinase is highly expressed and widely distributed in most tested human tissues except in the brain and spinal cord, where it is more moderately expressed. Orthologues of HIPK3 are highly conserved in vertebrates and insects. HIPK3 is involved in apoptosis (1). It interacts with Nkx1-2. JNK regulates the expression of HIPK3 in prostate cancer cells and this contributes to increased resistance to Fas receptor-mediated apoptosis by reducing the interaction between FADD and caspase-8 (2). HIPK3 has been reported to phosphorylate FADD and is implicated in multidrug resistance in a number of tumors. HIPK3 increases transcription factor SF-1 activity leading to increased steroidogenic gene expression in response to cAMP signaling.