Product Name: KinSub9RRGSF
Product Number: PE-01ALP95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub9RRGSF
Product Use: For assaying the phosphotransferase activity of cAMP-dependent protein kinase catalytic subunit beta (PKACb, UniProt ID P22694). The KinSub9RRGSF peptide demonstrated medium phosphotransferase activity with PKACb, and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub9RRGSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GFLSRRGSFGNGGHG
Product Use: For assaying the phosphotransferase activity of cAMP-dependent protein kinase catalytic subunit beta (PKACb, UniProt ID P22694). The KinSub9RRGSF peptide demonstrated medium phosphotransferase activity with PKACb, and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub9RRGSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GFLSRRGSFGNGGHG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1504.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: PKACb
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1504.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: PKACb
Scientific Background: PKACb is one of several protein kinases that can phosphorylate KinSub9RRGSF. Human PKACb (also known as PRKACB) is a protein-serine/threonine kinase of 351 amino acid length, with a predicted molecular mass of 40,623 Da. It is a member of the AGC group of protein kinases in the PKA family. It is highly expressed and widely distributed in most tested human tissues. Orthologues of PKACb are amongst the most highly conserved protein kinases in animals, plants, fungi and unicellular eukaryotes. It is activated by binding of two cAMP molecules to each of the two associated regulatory subunits in the PKA holoenzyme. Binding of cAMP induces dissociation of the two active catalytic subunits. Phosphorylation of T198 increases phosphotransferase activity. Phosphorylation of S339 plays a key role in stabilizing PKA. PKACb has been assigned to human chromosome region 1p36.1 (1). At the amino acid level C-alpha and C-beta showed 93% homology.
© Kinexus Bioinformatics Corporation 2017