Product Name: KinSub9RRKSF
Product Number: PE-01ALQ95
Size: | 200 µg | | Price: | 99.00 |
| | | $US | |
Peptide Name: KinSub9RRKSF
Product Use: For assaying the phosphotransferase activity of Mammalian STE20-like protein-serine kinase 3; Serine-threonine-protein kinase 24 (MST3, UniProt ID Q9Y6E0). The KinSub9RRKSF peptide demonstrated high phosphotransferase activity with Pim1, and exhibited very low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub9RRKSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRSRRKSFRRIGHG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1726 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Pim1
Scientific Background: MST3 is one of several protein kinases that can phosphorylate KinSub9RRKSF. Human Mst3 is a protein-serine/threonine kinase of 443 amino acid length, with a predicted molecular mass of 49,308 Da. It is a member of the STE group of protein kinases in the STE20 family, and YSK subfamily. This kinase is highly expressed and widely distributed in most tested human tissues. Orthologues of Mst3 are highly conserved in animals and plants. MST3 contains a conserved kinase domain at its N-terminus and a regulatory domain at its C-terminus. Caspase-mediated cleavage of the regulatory domain of MST3 activates its intrinsic kinase activity and leads to nuclear translocation. Expression of C-terminal truncated MST3 in cells results in DNA fragmentation and induction of apoptosis (1). MST3 can inhibit cell migration in a fashion dependent on autophosphorylation and can regulate paxillin phosphorylation through tyrosine phosphatase PTP-PEST (2).
References[1] Huang CY, Wu YM, Hsu CY, Lee WS, Lai MD, Lu TJ, Huang CL, Leu TH, Shih HM, Fang HI, Robinson DR, Kung HJ, Yuan CJ. Caspase activation of mammalian sterile 20-like kinase 3 (Mst3). Nuclear translocation and induction of apoptosis. J Biol Chem. 2002 Sep 13;277(37):34367-74. Epub 2002 Jul 9. PMID:12107159[2] Lu TJ, Lai WY, Huang CY, Hsieh WJ, Yu JS, Hsieh YJ, Chang WT, Leu TH, Chang WC, Chuang WJ, Tang MJ, Chen TY, Lu TL, Lai MD. Inhibition of cell migration by autophosphorylated mammalian sterile 20-like kinase 3 (MST3) involves paxillin and protein-tyrosine phosphatase-PEST. J Biol Chem. 2006 Dec 15;281(50):38405-17. Epub 2006 Oct 17. PMID:17046825