Product Name: ZIPtide KinSub
Product Number: PE-01ANB95
Size: 200 µg      Price:42.00
1 mg      $US83.00
5 mg      182.00
Peptide Name: ZIPtide KinSub

Product Use: For assaying the phosphotransferase activity of Death-associated protein kinase 3 (DAPK3, UniProt ID O43293).

Peptide Production Method: Solid-phase peptide synthesis

Peptide Sequence: KKLNRTLSFAEPG

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 1459.7 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: PASK; DAPK3 (ZIPK); CAMK1

Scientific Background: DAPK3 (also known as ZIP) is a protein-serine/threonine kinase that is a member of the CAMK group of protein kinases in the DAPK family. This kinase is moderate to highly expressed in most tested human tissues. Orthologues of DAPK3 are highly conserved in animals and plants. DAPK3 is activated by phosphorylation at T180, T225, and T265. Homodimers of DAPK3 or heterodimers with ATF4 formed via a leucine zipper domain is required for full enzymatic activity. DAPK3 plays a role in apoptosis. It phosphorylates core histones H3 and H4, and myosin light chain in vitro. DAPK3 interacts with transcription and splicing factors as well as with pro-apoptotic protein Par-4.
References

[1] Preuss U, Landsberg G, Scheidtmann KH. Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinase. Nucleic Acids Res. 2003 Feb 1;31(3):878-85. PMID: 12560483.

[2] Kawai T, Matsumoto M, Takeda K, Sanjo H, Akira S. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol Cell Biol. 1998 Mar;18(3):1642-51. PMID: 9488481.