Product Name: MRCKb (408-427)
Product Number: PE-01BEE65
| Size: | 200 µg | Price: | 14.00 | |
| 1 mg | $US | 29.00 |
Peptide Name: MRCKb (408-427)
Product Use: Services as a blocking peptide for use with the MRCKb-AKCD rabbit polyclonal antibody (Cat. No.: AB-NK283-1) that is also available from Kinexus. The peptide sequence is located right after the kinase catalytic domain.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: CFSDRGSLKSIMQSNTLTKD
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 2230.5 Da
Peptide Purity Percent after Synthesis and Purification: >50
Product Use: Services as a blocking peptide for use with the MRCKb-AKCD rabbit polyclonal antibody (Cat. No.: AB-NK283-1) that is also available from Kinexus. The peptide sequence is located right after the kinase catalytic domain.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: CFSDRGSLKSIMQSNTLTKD
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 2230.5 Da
Peptide Purity Percent after Synthesis and Purification: >50
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: MRCKb pan-specific antibody (Cat. No.: AB-NK283-1)
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: MRCKb pan-specific antibody (Cat. No.: AB-NK283-1)
Scientific Background: MRCK-ß (CDC42-binding protein kinase) is a member of the AGC group of protein kinases in the DMPK family, and GEK subfamily. This kinase is moderate to highly expressed in most tested human tissues. The MRCK-ß protein posesses many functional domains, including three independent coiled-coil (CC) domains involved in the dimerization that is critical for the activation of the protein, a cysteine-rich motif similar to that of protein kinase C (PKC), and potentially a pleckstrin homology (PH) domain. The two distal CC domains (CC2 and CC3) participate in the autoinhibition of the kinase catalytic domain of the protein. The kinase activity of the protein can be elevated by treatment of the cells with phorbol ester, which has been shown to bind to a cysteine-rich domain close to the distal CC domain of the protein. MRCKb interacts tightly with GTP-bound but not GDP-bound CDC42 and RhoA, and this permits N-terminus-mediated dimerization and activation of the phosphotransferase activity of MRCKb by transautophosphorylation. MRCKb mediates RhoA and Cdc42 regulation of actin cytoskeletal reorganization and neurite outgrowth. MRCK-ß has a key role in cytoskeletal reorganization and cell migration. MRCK-ß directly phosphorylates PPP1R12C and MYL9/MLC2 to stimulate actin cytoskeletal reorganization. In addition, the protein acts in combination with MYO18A and LURAP1 to modulate lamellar actomyosin retrograde flow, which is critical to the protrusion of cellular processes and cell migration. Furthermore, the direct phosphorylation of myosin light chain at S19 by MRCK-ß is necessary for actin-myosin contractility. Effects of the repeat expansion on the MRCKb gene may be responsible for muscle and heart features of Myotonic Dystrophy. MRCKb has also been linked with the development of breast (ductal), colorectal and lung large cell carcinomas.
© Kinexus Bioinformatics Corporation 2017