Product Name: BrskSubtide
Product Number: PE-01BGL99
Size: 200 µg      Price:49.00
1 mg      $US97.00
5 mg      213.00
Peptide Name: BrskSubtide

Product Use: For assaying the phosphotransferase activity of BR serine/threonine-protein kinase 2 (UniProt ID Q8IWQ3).

Peptide Production Method: Solid-phase peptide synthesis

Peptide Sequence: KRERRKSFRPFLG

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: βAla-Cys

Peptide Molecular Mass Calculated: 1851.4 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: Brsk

Scientific Background: BRSK1 is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It is activated by phosphorylation on T205 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Its substrates include CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Its phosphotransferase activity is lost with the K59A, and G327A mutations. It is involved in the UV-induced DNA damage checkpoint response, likely by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C. A LKB1/LKB1 complex with the STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 phosphorylates and activates BRSK1, but this can be prevented by an inhibitory T189A mutation.