Product Name: LckSubtide
Product Number: PE-01BHV90
Size: | 200 µg | | Price: | 45.00 |
| 1 mg | | $US | 91.00 |
| 5 mg | | | 214.00 |
Peptide Name: LckSubtide
Product Use: For assaying the phosphotransferase activity of Lymphocyte-specific protein-tyrosine kinase; Proto-oncogene tyrosine-protein kinase LCK (UniProt ID P06239).
Peptide Production Method: Solid-phase peptide synthesis
Peptide Sequence: KKEEDDDYENLEEA
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Molecular Mass Calculated: 1900.86 Da
Peptide Purity Percent after Synthesis and Purification: >90
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Lck
Scientific Background: Lck is a non-receptor protein-tyrosine kinase of the TK group and Src family. Phosphorylation of Y394 increases phosphotransferase activity and induces interaction with Lck. Phosphorylation of Y192 and Y505 inhibits Lck phosphotransferase activity. Lck has a critical function in the selection and maturation of developing T-cells in the thymus, as well as in the function of mature T-cells throughout the body. Lck is a key signalling component of the T-cell antigen receptor (TCR)-linked signal transduction pathway and is constitutively bound to the cytoplasmic domains of the CD4 and CD8 membrane receptors. Lck is expressed through all stages of thymocyte development and is necessary for regulating both pre-TCR and mature alpha beta TCR-mediated maturation. Lck may be an oncoprotein (OP) based on its similarity to other Src family protein-tyrosine kinases.