Product Name: PKASubtide
Product Number: PE-01BIF95
Size: 200 µg      Price:36.00
1 mg      $US72.00
5 mg      157.00
Peptide Name: PKASubtide

Product Use: For assaying the phosphotransferase activity of cAMP-dependent protein kinase catalytic subunit beta (UniProt ID P22694).

Peptide Production Method: Solid-phase peptide synthesis

Peptide Sequence: RRRRGSLVG

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: βAla-Cys

Peptide Molecular Mass Calculated: 1230.45 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid
Storage Conditions: -20°C

Peptide Recommended Enzyme: PKA

Scientific Background: PKACb (also known as PRKACB) is a protein-serine/threonine kinase that is a member of the AGC group of protein kinases in the PKA family. It is highly expressed and widely distributed in most tested human tissues. Orthologues of PKACb are amongst the most highly conserved protein kinases in animals, plants, fungi and unicellular eukaryotes. It is involved in mediating cell cycle, cell proliferation, cell differentiation, microtubule characteristics, condensation of chromatin, de-condensation of chromatin, nuclear envelope deassembly and reassembly, and ion flux. It is activated by binding of two cAMP molecules to each of the two associated regulatory subunits in the PKA holoenzyme. Binding of cAMP induces dissociation of the two active catalytic subunits. Phosphorylation of T198 increases phosphotransferase activity. Phosphorylation of S339 plays a key role in stabilizing PKA. At the amino acid level C-alpha and C-beta showed 93% homology.