Product Name: S6KASubtide
Product Number: PE-01BJH95
Size: 200 µg      Price:42.00
1 mg      $US84.00
5 mg      185.00
Peptide Name: S6KASubtide

Product Use: For assaying the phosphotransferase activity of Ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase 1 (UniProt ID P23443).

Peptide Production Method: Solid-phase peptide synthesis

Peptide Sequence: RRRRDSFCFFG

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: βAla-Cys

Peptide Molecular Mass Calculated: 1619.89 Da

Peptide Purity Percent after Synthesis and Purification: 95

Peptide Appearance: White powder

Peptide Form: Solid
Storage Conditions: -20°C

Peptide Recommended Enzyme: S6KA

Scientific Background: p70S6K (RPS6KB1) is a protein-serine/threonine kinase that is a member of the AGC group of protein kinases in the RSK family, and p70 subfamily. This kinase is highly expressed and widely distributed in most tested human tissues except in the brain and spinal cord where it is more moderately expressed. It functions downstream of mTOR (FRAP1) signalling in response to growth factors to promote cell proliferation, growth, and progression through the cell cycle. p70S6K is activated by phosphorylation at T252 (by PDK1), S394 (by mTOR), T412 (by mTOR), S427, S434 (by ERK1/2), S441, T444 (by ERK1/2) and S447 (by ERK1/2). p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6. It is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K phosphotransferase activity changes during the cell cycle, and increases 20-fold in G1 cells released from G0. It has been linked with the development of diet-induced obesity, colon adenocarcinoma and breast cancer.