Product Name: S6KBSubtide
Product Number: PE-01BJI85
Size: 200 µg      Price:32.00
1 mg      $US63.00
5 mg      137.00
Peptide Name: S6KBSubtide

Product Use: For assaying the phosphotransferase activity of Ribosomal protein S6 kinase beta-2; Ribosomal protein S6 kinase 2 (UniProt ID Q9UBS0).

Peptide Production Method: Solid-phase peptide synthesis

Peptide Sequence: RRRRKSFRRFG

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: βAla-Cys

Peptide Molecular Mass Calculated: 1695.04 Da

Peptide Purity Percent after Synthesis and Purification: >80

Peptide Appearance: White powder

Peptide Form: Solid
Storage Conditions: -20°C

Peptide Recommended Enzyme: S6KB

Scientific Background: p70S6Kb (RPS6KB2) is a protein-serine/threonine kinase that is a member of the AGC group of protein kinases in the RSK family, and p70 subfamily. It acts downstream of mTOR signalling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. It controls protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including EIF2B and EIF4B. It also regulates translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, and targeting it for ubiquitination and subsequent proteolysis. It promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, it activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and activation of EEF2. It negatively regulates mTORC2 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signalling. It mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. It phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. It mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues to accelerated degradation of IRS1.