Product Name: TrkSubtide
Product Number: PE-01BJQ95
Size: 200 µg      Price:52.00
1 mg      $US104.00
5 mg      227.00
Peptide Name: TrkSubtide

Product Use: For assaying the phosphotransferase activity of Nerve growth factor (NGF) receptor-tyrosine kinase; High affinity nerve growth factor receptor (UniProt ID P04629).

Peptide Production Method: Solid-phase peptide synthesis

Peptide Sequence: KKGIEDDDYVNPGG

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: βAla-Cys

Peptide Molecular Mass Calculated: 1679.76 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: Trk

Scientific Background: TrkA (NTRK1) is a protein-tyrosine kinase of the TK group and Trk family. It is a receptor protein-tyrosine kinase with high affinity for nerve growth factor (NGF). The TrkA protein functions in the development and maturation of both the central nervous system (CNS) and peripheral nervous system (PNS) through the regulation of neuronal proliferation, differentiation, and survival, specifically of sympathetic neurons. Upon binding to NGF, TrkA undergoes homodimerization and activating autophosphorylation. Autophosphorylation of Y496 increases phosphotransferase activity and induces interaction with FRS2 and Shc1. Autophosphorylation of Y757 increases phosphotransferase activity. Autophosphorylation of Y791 increases phosphotransferase activity and induces interaction with PLCg1. Phosphorylation of Y701 inhibits phosphotransferase activity and induces interaction with CLTC and LAMP1. These interactions mediate NGF effects on cell survival and proliferation. TrkA appears to be an oncoprotein (OP). Overactive forms of the TrkA protein are known to have oncogenic properties and promote tumourigenesis. Chromosomal translocations involving the TrkA gene have been observed in thyroid papillary carcinoma tissue samples.