Product Name: Nek2 (168-174) pT170+pS171
Product Number: PE-04ABO90
| Size: | 200 µg | Price: | 54.00 | |
| 1 mg | $US | 108.00 | ||
| 5 mg | 253.00 |
Peptide Name: Nek2 (168-174) pT170+pS171
Product Use: Services as a blocking peptide for use with the Nek2-pT170+pS171 rabbit polyclonal antibody (Cat. No.: AB-PK733) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T170 and S171 stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: HD-pT-pS-FAK
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Product Use: Services as a blocking peptide for use with the Nek2-pT170+pS171 rabbit polyclonal antibody (Cat. No.: AB-PK733) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T170 and S171 stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: HD-pT-pS-FAK
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1180.1 + 1138 Da
Peptide Purity Percent after Synthesis and Purification: >90
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: Nek2 - pT170+pS171 phosphosite-specific antibody (Cat. No.: AB-PK733)
Peptide Molecular Mass Calculated: 1180.1 + 1138 Da
Peptide Purity Percent after Synthesis and Purification: >90
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: Nek2 - pT170+pS171 phosphosite-specific antibody (Cat. No.: AB-PK733)
Scientific Background: Nek2 (NIMA) is a protein-serine/threonine kinase of the Other group and Nek family. It regulates bipolar spindle formation and centrosome separation in mitotic cells, and affects chromatin condensation in meiotic cells. Nek2 can phosphorylate CROCC, CEP250 and NINL, ultimately removing their ability to form a complex in the centrosomes. The proteins mitotic checkpoint proteins CDC20 and MAD2L1 are also phosphorylated and mediated by Nek2. The effect on chromosome condensation is via HMGA2. The ability for Nek2 to activate Nek11 is lost with a K37R mutation. Nek2 kinase phosphotransferase activity can be increased two-fold with a T170E, or S171D, or T175E mutation, while the phosphotransferase activity can be decreased two-fold with a T175A mutation. Complete kinase phosphotransferase activity can be lost with a T179A, T179E, S241A, or S241D mutations. Nek2 is linked to Plexiform Neurofibromas, and chromosome polyploidy. Polyploidy is linked to breast cancer and melanoma, and most often affects smooth muscle, liver, and breast tissues. Nek2 dysfunction has been associated with both primary and secondary breast tumours.
© Kinexus Bioinformatics Corporation 2017