Product Name: Btk (220-226) pY223+pY225
Product Number: PE-04ACL90
Size: 200 µg      Price:53.00
1 mg      $US107.00
5 mg      231.00
Peptide Name: Btk (220-226) pY223+pY225

Product Use: Services as a blocking peptide for use with the Btk-pY223+pY225 rabbit polyclonal antibody (Cat. No.: AB-PK550) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the SH3_1 domain. Y223 and Y225 phosphorylation stimulate phosphotransferase activity and regulates cell growth.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: Homo sapiens

Peptide Sequence: VAL-pY-D-pY-M

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: βAla-Cys

Peptide Modifications Other: Phosphorylated

Peptide Molecular Mass Calculated: 1208.17 Da

Peptide Purity Percent after Synthesis and Purification: >80

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Related Product 1: Btk - pY223+pY225 phosphosite-specific antibody (Cat. No.: AB-PK550)

Scientific Background: Btk is a protein-tyrosine kinase of the TK group and Tec family. It is a non-receptor kinase that is essential for B-cell development, differentiation, and function. In addition, Btk phosphotransferase activity is required for radiation induced apoptosis in immune cells. After B-cell receptor (BCR) activation, Btk translocates to the plasma membrane and is phosphorylated on Y551 catalyzed by the Lyn and Syk proteins, followed by autophosphorylation at Y223. Phosphorylation at Y223 and Y551 increases phosphotransferase activity.Phosphorylation at S180 causes translocation back to the cytoplasm, leading to downstream phosphorylation of PLCG2 at several conserved phosphosites and the stimulation of downstream calcium dependent signalling mechanisms. Btk is thought to act as a scaffold to bring together various intracellular signalling proteins to function in cytokine receptor signalling pathways. In immune cells, Btk is a vital component of the toll-like receptor (TLR) pathway, which is the primary system used to identify pathogens and activate host defence systems.