Product Name: p70S6Kb (420-426) pS423
Product Number: PE-04AGI95
| Size: | 200 µg | Price: | 42.00 | |
| 1 mg | $US | 84.00 | ||
| 5 mg | 185.00 |
Peptide Name: p70S6Kb (420-426) pS423
Product Use: Services as a blocking peptide for use with the p70S6KB-pS423 rabbit polyclonal antibody (Cat. No.: AB-PK747) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the C-terminal fifth of the kinase. S423 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: APV-pS-PLK
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Product Use: Services as a blocking peptide for use with the p70S6KB-pS423 rabbit polyclonal antibody (Cat. No.: AB-PK747) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the C-terminal fifth of the kinase. S423 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: APV-pS-PLK
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 965.06 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: p70S6Kb - pS423 phosphosite-specific antibody (Cat. No.: AB-PK747)
Peptide Molecular Mass Calculated: 965.06 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: p70S6Kb - pS423 phosphosite-specific antibody (Cat. No.: AB-PK747)
Scientific Background: p70S6Kb (RPS6KB2) is a protein-serine/threonine kinase that is a member of the AGC group of protein kinases in the RSK family, and p70 subfamily. It acts downstream of mTOR signalling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. It controls protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including EIF2B and EIF4B. It also regulates translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, and targeting it for ubiquitination and subsequent proteolysis. It promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, it activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and activation of EEF2. It negatively regulates mTORC2 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signalling. It mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. It phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. It mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues to accelerated degradation of IRS1.
© Kinexus Bioinformatics Corporation 2017