Product Name: AMPKa1 (180-186) pT183+pS184
Product Number: PE-04AIG99
| Size: | 200 µg | Price: | 61.00 | |
| 1 mg | $US | 123.00 | ||
| 5 mg | 269.00 |
Peptide Name: AMPKa1 (180-186) pT183+pS184
Product Use: Services as a blocking peptide for use with the AMPKa1-pT183+pS184 rabbit polyclonal antibody (Cat. No.: AB-PK521) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T183 and S184 stimulate phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: FLR-pS-pT-CG
Peptide Modifications N Terminus: Free amino
Product Use: Services as a blocking peptide for use with the AMPKa1-pT183+pS184 rabbit polyclonal antibody (Cat. No.: AB-PK521) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T183 and S184 stimulate phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: FLR-pS-pT-CG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1116.1 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: AMPKa1 - pT183+pS184 phosphosite-specific antibody (Cat. No.: AB-PK521)
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1116.1 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: AMPKa1 - pT183+pS184 phosphosite-specific antibody (Cat. No.: AB-PK521)
Scientific Background: AMPKa1 (PRKAA1) is a protein-serine/threonine kinase of the CAMK group and CAMKL family. AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits. Binding of AMP results in allosteric activation, inducing phosphorylation on T183 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signalling pathways in response to adiponectin, leptin and CAMKK-beta. Phosphorylation at T174 by CAMKK1 and CAMKK2 increases phosphotransferase activity, triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.
© Kinexus Bioinformatics Corporation 2017