Product Name: Fyn (528-534) pY531
Product Number: PE-04AKJ90
| Size: | 200 µg | Price: | 35.00 | |
| 1 mg | $US | 71.00 | ||
| 5 mg | 153.00 |
Peptide Name: Fyn (528-534) pY531
Product Use: Services as a blocking peptide for use with the Fyn-pY531 rabbit polyclonal antibody (Cat. No.: AB-PK645) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the C-terminus of the kinase. This is the major in vivo phosphorylation sites in Fyn. Phosphorylation induces Inhibition of the phosphotransferase activity of Fyn.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: EPQ-pY-QPG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Product Use: Services as a blocking peptide for use with the Fyn-pY531 rabbit polyclonal antibody (Cat. No.: AB-PK645) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the C-terminus of the kinase. This is the major in vivo phosphorylation sites in Fyn. Phosphorylation induces Inhibition of the phosphotransferase activity of Fyn.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: EPQ-pY-QPG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1072.03 Da
Peptide Purity Percent after Synthesis and Purification: >80
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: Fyn - pY531 phosphosite-specific antibody (Cat. No.: AB-PK645)
Peptide Molecular Mass Calculated: 1072.03 Da
Peptide Purity Percent after Synthesis and Purification: >80
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: Fyn - pY531 phosphosite-specific antibody (Cat. No.: AB-PK645)
Scientific Background: Fyn is a protein-tyrosine kinase of the TK group and Src family. It is a non-receptor kinase that mediates cell growth, survival, and adhesion. This kinase is highly expressed and widely distributed in most tested human tissues. Fyn is inhibited by phosphorylation at Y531. Fyn has been shown to phosphorylate Dab1, an intracellular adaptor protein that interacts with amyloid precursor protein (APP) and apoE receptor 2 (apoEr2). The interaction of Fyn and Dab1 regulates the phosphorylation, trafficking, and processing of APP and apoEr2. Fyn can interact with Fyn-binding protein and the p85 subunit of PI3K. It has been shown to be part of a protein complex involving DCC and FAK, which is downstream of Netrin-1, a pathway important in promoting both axonal outgrowth and axonal guidance in path finding. Fyn may be an oncoprotein (OP) based on its similarity to other Src family kinases that are known oncoproteins. Fyn expression has been shown to be significantly increased in Chronic Myelogenous Leukemia (CML). Knockdown of Fyn with shRNA slows leukemia cell growth, inhibits clonogenicity, and leads to increased sensitivity to imatinib. Fyn has also been linked with the development of lung squamous cell carcinomas (LSCC), melanomas (metastatic) and epilepsy. The active form of the protein kinase normally acts to promote tumour cell proliferation. Fyn has been linked with estrogen-receptor negative breast cancer, which is related to breast and colon cancer, and it may affect breast, lymph node, or lung tissues. Insertional mutagenesis studies in mice also support a role for this protein kinase in mouse cancer oncogenesis.
© Kinexus Bioinformatics Corporation 2017