Product Name: BARK1 (347-359) pS350+pT353+pY356
Product Number: PE-04ARY40
Size: 200 µg      Price:23.00
1 mg      $US46.00
5 mg      124.00
Peptide Name: BARK1 (347-359) pS350+pT353+pY356

Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. Phosphorylation of the S350 site is predicted to be stimulatory, whereas the T353 and Y356 sites are predicted to be inhibitory.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: Homo sapiens

Peptide Sequence: PHA-pS-VG-pT-HG-pY-MAP

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: βAla-Cys

Peptide Modifications Other: Phosphorylated

Peptide Molecular Mass Calculated: 1737.6 Da

Peptide Purity Percent after Synthesis and Purification: 43.2

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Scientific Background: BARK1 (GRK2, ADRBK1) is a protein-serine/threonine kinase of the AGC group and GRK family. It is activated by G-beta/gamma subunit complex. Phosphorylation of S29 increases phosphotransferase activity. Phosphorylation of S670 inhibits G-beta/gamma-mediated activation of GRK2. Phosphorylation of Y13, Y86, Y92 and S685 increases interaction with G-alpha(q) and may induce protein degradation.