Product Name: CDK1 (10-23) pT14+pY15+pY19
Product Number: PE-04ASD90
Size: 200 µg      Price:47.00
1 mg      $US94.00
Peptide Name: CDK1 (10-23) pT14+pY15+pY19

Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The presentation as a dimer increases the potential activity. The peptide sequence is located in the protein kinase catalytic domain in subdomain I, which is involved in binding the phosphates in the ATP substrate. Phosphorylation inhibits phosphotransferase activity. Phosphorylation regulates cell cycle progression.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: Homo sapiens

Peptide Sequence: (IGEG-pT-pY-GVV-pY-KGRH-βA-Cys)2

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Amide

Peptide Modifications Other: Phosphorylated, dimer

Peptide Molecular Mass Calculated: 3979.8 Da

Peptide Purity Percent after Synthesis and Purification: ~50

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Storage Stability: Stable as dimer

Scientific Background: CDK1 (CDC2) is a protein-serine/threonine kinase of the CMGC group and CDK family. It plays an essential role in cell cycle control in eukaryotic cells by regulating the centrosome cycle, mitotic onset, G2-M phase transition, G1 progression, and G1-S phase transition through an association with various interphase cyclin proteins. Phosphorylation events at T14 or Y15 on the protein are inactivating, while phosphorylation at T161 is stimulatory. CDK1 appears to be a tumour requiring protein (TRP). Gain-of-function mutations in the CDK1 gene have been linked to several forms of cancer, indicating an oncogenic role for the CDK1 protein. Cells transformed with the oncogene MYC undergo apoptosis when treated with small-molecule CDK1 inhibitors. Elevated expression of CDK1 has been reported as a diagnostic marker for cancer progression in esophageal adenocarcinoma, potentially reflecting the role of the CDK1 protein in tumourigenesis. CDK1 expression can be used as a prognostic indicator for early breast cancer. For example, breast cancer tumours with high expression of CDK1 are correlated with a significantly lower 5-year patient survival rate (66. 9%) than tumours that have low levels of CDK1 expression (84. 2%).