Product Name: HIPK1 (353-360) pS355+pY357+pY358
Product Number: PE-04AUU01
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Peptide Name: HIPK1 (353-360) pS355+pY357+pY358
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. These are the major in vivo phosphorylation sites in HIPK1. S355, Y357 and Y358 phosphorylation are predicted to be inhibitory for phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: LQ(pS)R(pY)(pY)RA
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. These are the major in vivo phosphorylation sites in HIPK1. S355, Y357 and Y358 phosphorylation are predicted to be inhibitory for phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: LQ(pS)R(pY)(pY)RA
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Modifications Other: Phosphorylated
Storage Conditions: -20°C
Storage Conditions: -20°C
Scientific Background: HIPK1 (HIK1) is a protein-serine/threonine kinase of the CMGC group and DYRK family. It functions in transcription regulation as well as tumour necrosis factor (TNF)-mediated apoptosis. HIPK1 is also known to act as a co-repressor for homeodomain transcription factors. In the absence of TNF, HIPK1 prevents MAP3K5-JNK activation. When present, TNF induces nuclear translocation of HIPK1, thus removing it from the cytoplasm, resulting in the activation of MAP3K5-JNK signalling by de-repression. In addition, through the direct phosphorylation of p53 at the S15 residue, HIPK1 activates p53 leading to increased p21 protein levels, which subsequently inhibits cell proliferation. HIPK1 may be a tumour suppressor protein (TSP). Over-expression of HIPK1 has been observed in colorectal cancer specimens, with highest levels at the beginning of tumourigenesis followed by a progressive reduction over time as the cancer develops, indicating a role for the HIPK1 protein in the early events of tumourigenesis. in addition, these specimens also displayed native p53 and normal levels of p21, indicating that the HIPK1 protein functions as a tumour suppressor and that a loss-of-function in the protein is associated with tumourigenesis.
© Kinexus Bioinformatics Corporation 2017