Product Name: Arg (436-442) pY439
Product Number: PE-04AYK00
| Size: | 200 µg | Price: | 30.00 | |
| $US |
Peptide Name: Arg (436-442) pY439
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases and for epitope mapping of phosphosite-specific antibodies. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. Y439 phosphorylation is predicted to be stimulatory for phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: GDT-pY-TAH
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Modifications Other: Phosphorylated
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases and for epitope mapping of phosphosite-specific antibodies. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. Y439 phosphorylation is predicted to be stimulatory for phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: GDT-pY-TAH
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 842.72 Da
Peptide Purity Percent after Synthesis and Purification: >80
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Purity Percent after Synthesis and Purification: >80
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Scientific Background: Arg (Abl2) is a protein-tyrosine kinase of the TK group and Abl family. It features SH3 and SH2 domains, a protein- tyrosine kinase domain, and a nuclear localization domain. It shares many functions with Abl1, which includes cytoskeleton remodelling through phosphorylation of enzymes responsible for cytoskeleton rearrangements for signalling, adhesion, and movement as well as microtuble subunits, TUBA1 and TUBB. It also contains F-actin binding region to bundle and regulate actin cytoskeletal structure. It possesses autocatalytic activity and the ability to phosphorylate its inhibitor ABI1 to regulate itself. Arg gene expression is found in both normal and tumour cells, it is often found as a fusion gene with ETV6 in acute non lymphocytic leukemias. However, the broad distribution of point mutations seen in various cancers, including about a third within the kinase catalytic domain, indicate that these mutations are likely to be silent or result in loss of function. Arg has also been reported to promote breast tumour cell invasion and dissemination, but attenuates the growth of breast tumours. This indicates that wild-type Arg may as a tumour suppressor protein. Phosphorylation of Tyr-261 reduces ubiquitination and protein degradation. Arg is activated by autophosphorylation as well as by Src-family kinase-mediated phosphorylation. It is also activated by RIN1 binding to the SH2 and SH3 domains.
© Kinexus Bioinformatics Corporation 2017