Product Name: MKK4 (254-260) pS257
Product Number: PE-04BAD00
| Size: | 200 µg | Price: | 22.00 | |
| $US |
Peptide Name: MKK4 (254-260) pS257
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases and for epitope mapping of phosphosite-specific antibodies. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S257 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: LVD-pS-IAK
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Modifications Other: Phosphorylated
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases and for epitope mapping of phosphosite-specific antibodies. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S257 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: LVD-pS-IAK
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 823.86 Da
Peptide Purity Percent after Synthesis and Purification: >70
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Storage Stability: stable
Peptide Purity Percent after Synthesis and Purification: >70
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Storage Stability: stable
Scientific Background: MKK4 (MAP2K4, MEK4) is a dual specificity protein kinase of the STE group and STE7 family Phosphorylation of S257 and T261 increases phosphotransferase activity. Phosphorylation of S80 inhibits its phosphotransferase activity. It acts as a critical component in the MAP kinase (MAPK) and the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signalling pathways. It has a role in maintaining peripheral lymphoid homeostasis and apoptosis. Mutations and deletions of this genes leads to dysregulated JNK signalling and promotes oncogenesis. MKK4 appears to be a tumour suppressor protein (TSP), but it may also promote metastasis. Cancer-related mutations in human tumours point to a loss of function of the protein kinase. The active form of the protein kinase normally acts to inhibit tumour cell proliferation. However, significantly elevated expression of MKK4 has been observed in invasive pancreatic cancer specimens, indicating a possible role for the protein in tumourigenesis. MKK4 over-expression is correlated with increased expression of heat shock protein 27 (HSP27) and matrix metalloproteinase 2 (MMP-2) protein, which are required for the increased invasive phenotype of the cells. Therefore, MKK4 appears to have an important role in the promotion of metastasis in pancreatic cancer cells. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis.
© Kinexus Bioinformatics Corporation 2017