Product Name: Phosphotyrosine
Product Number: AB-CN005-2
Size: 25 µg      Price:89.00
      $US
Target Full Name: Phosphotyrosine sites in proteins

Target Alias: PhosphoTyrosine (pY); Phospho-Tyrosine

Product Type Specific: Generic phosphotyrosine-specific pan-specific antibody

Antibody Code: CN005-2

Antibody Target Type: Pan-specific

Antibody Type: Monoclonal

Antibody Host Species: Mouse

Antibody Ig Isotype Clone: IgG1

Antibody Immunogen Source: Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin with 1-ethyl-3-(3'-dimentrylaminopropyl) carbodiimide

Production Method: Protein G purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: Pan-specific

Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation

Antibody Dilution Recommended: WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.

Antibody Potency: Medium potency. Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in both unstimulated and stimulated cell lysates.

Antibody Species Reactivity: Species Independent

Antibody Positive Control: 1 µg/ml of SMC-174 was sufficient for detection of phosphorylated tyrosine residues in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

Antibody Specificity: High

Antibody Cross Reactivity: Does not cross-react with phosphoserine or phosphothreonine.

Related Product 1: PYK generic phosphotyrosine-specific antibody (Cat. No.: AB-PG001)

Related Product 2: PYK-Ultra generic phosphotyrosine-specific antibody (Cat. No.: AB-PG001U)

Scientific Background: Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (3). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (4-6). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (7). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (8).
References

[1] Garton A.J., Flint A.J., and Tonks N.K. (1996) Mol and Cell Bio 16(11): 6408-6418.

[2] Garton A.J., and Tonks N.K. (1999) J Bio Chem. 274(6): 3811-3818.

[3] Goto H. et al. (2005) Nature Cell Biology 8: 180-187.

[4] Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365.

[5] Downward J. (2001) Nature 411: 759-762.

[6] Pawson T. and Saxton T.M. (1999) Cell 97: 675-678.

[7] Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352.

[8] Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656.
[9] Tiganis T., Kemp B.E., and Tonks N.K. (1999) J. Bio Chem. 274(39): 27768-27775.