Product Type Specific: Generic phosphotyrosine-specific antibody

Antibody Code: PG001U

Antibody Target Type: Generic phosphotyrosine-specific

Antibody Phosphosite: Generic phospho

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Over 200 diverse phosphotyrosine phosphorylation site peptides

Antibody Immunogen Description: Diverse locations

Production Method: Over 400 immunizing phosphopeptides were produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purities were assessed by analytical hplc and the amino acid sequences confirmed by mass spectrometry analysis. These peptides were coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto agarose columns to which a phosphotyrosine-beta-alanine-cysteine was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris. The antibody was also subjected to negative purification over a rabbit IgG-agarose column.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Related Product 1: PYK generic phosphotyrosine-specific antibody (Cat. No.: AB-PG001)

Related Product 2: Phosphotyrosine pan-specific antibody (Cat. No.: AB-CN005-2)