Product Name: PYK-Ultra
Product Number: AB-PG001U
|Size:|| 25 µg|| ||Price:||89.00|
Product Type Specific: Generic phosphotyrosine-specific antibody
Antibody Code: PG001U
Antibody Target Type: Generic phosphotyrosine-specific
Antibody Phosphosite: Generic phospho
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Over 200 diverse phosphotyrosine phosphorylation site peptides
Antibody Immunogen Description: Diverse locations
Production Method: Over 400 immunizing phosphopeptides were produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purities were assessed by analytical hplc and the amino acid sequences confirmed by mass spectrometry analysis. These peptides were coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto agarose columns to which a phosphotyrosine-beta-alanine-cysteine was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris. The antibody was also subjected to negative purification over a rabbit IgG-agarose column.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.