Product Name: CaMK2g-PAD
Product Number: AB-NK020-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Calcium/calmodulin-dependent protein-serine kinase 2 gamma
Target Alias: Calcium/calmodulin-dependent protein kinase II gamma; CaM kinase II gamma subunit; CaMK2-gamma; CaMKG; CaMK-II gamma; CaMK-II gamma subunit; CaM-kinase II gamma; CaM-kinase II gamma chain; EC 2.7.11.17; Kinase CaMK2-gamma
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK020-1
Antibody Target Type: Pan-specific
Protein UniProt: Q13555
Protein SigNET: Q13555
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CaMK2g sequence peptide Cat. No.: PE-01BCJ90
Target Alias: Calcium/calmodulin-dependent protein kinase II gamma; CaM kinase II gamma subunit; CaMK2-gamma; CaMKG; CaMK-II gamma; CaMK-II gamma subunit; CaM-kinase II gamma; CaM-kinase II gamma chain; EC 2.7.11.17; Kinase CaMK2-gamma
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK020-1
Antibody Target Type: Pan-specific
Protein UniProt: Q13555
Protein SigNET: Q13555
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CaMK2g sequence peptide Cat. No.: PE-01BCJ90
Antibody Immunogen Sequence: CDGIKGSTESSNTTTEDEDL
Antibody Immunogen Description: Corresponds to amino acid residues D376 to L394; Before CaMAD, highly conserved in all CaMK2 isoforms
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues D376 to L394; Before CaMAD, highly conserved in all CaMK2 isoforms
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-60 kDa.
Related Product 1: CaMK2g-PAD blocking peptide
Related Product 2: CaMKII pT286/pT287 phosphosite-specific antibody (Cat. No.: AB-NK302-2)
Related Product 3: Autocamtide-2 peptide - Autocamtide 2 CaMK2-derived peptide substrate
Related Product 4: CAMK2Subtide - CaMK2a protein kinase substrate peptide
Related Product 5: Syntide-2 KinSub - Syntide-2 protein kinase substrate peptide
Related Product 1: CaMK2g-PAD blocking peptide
Related Product 2: CaMKII pT286/pT287 phosphosite-specific antibody (Cat. No.: AB-NK302-2)
Related Product 3: Autocamtide-2 peptide - Autocamtide 2 CaMK2-derived peptide substrate
Related Product 4: CAMK2Subtide - CaMK2a protein kinase substrate peptide
Related Product 5: Syntide-2 KinSub - Syntide-2 protein kinase substrate peptide
Scientific Background: CaMK2g is a protein-serine/threonine kinase of the CaMK group and CaMK2 family. It is a calcium/calmodulin-activated kinase that is involved in sarcoplasmic reticulum calcium transport, and may have a role in synaptic plasticity. Once activated by calcium or calmodulin CaMK2g will autophosphorylate at T287, and become activate in the absence of calcium.
Figure 1. Dot blotting CaMK2g-PAD antibody with recombinant purified proteins.
© Kinexus Bioinformatics Corporation 2017