Product Name: CaMKII pT286/pT287

Product Number: AB-NK302-2

Size:	25 µg		Price:	89.00
			$US

Target Full Name: Calcium/calmodulin-dependent protein kinase type II subunits

Target Alias: CaMK2; CaMK2A; CaMK2B; CaMK2D; CaMK2G; CAMKA

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: NK302-2

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T286

Protein UniProt: Q9UQM7

Protein SigNET: Q9UQM7

Antibody Type: Monoclonal

Antibody Host Species: Mouse

Antibody Ig Isotype Clone: IgG1

Antibody Immunogen Source: Synthetic peptide

Production Method: Protein G purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation | ELISA

Antibody Dilution Recommended: WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.

Antibody Potency: Detects a phosphorylated CaMKII from rat tissues. This antibody is specific for α and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β).

Antibody Species Reactivity: Human | Mouse | Rat

Antibody Positive Control: 1 µg/ml was sufficient for detection of 0.2µg CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Antibody Specificity: High

Related Product 1: CaMK2a-pT286 phosphosite-specific antibody (Cat. No.: AB-PK555)

Scientific Background: CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).

References

[1] Hughes K. et al. (2001) J. Biol. Chem. 276: 36008–3601

[2] Barria A. et al. (1997) Science 276: 2042–2045.

[4] Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808.

[5] Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479.

[6] Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102.

[7] Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292.

[8] Means A.R. (2000) Mol. Endocrinol. 14: 4–12.

[9] Makhinson M. et al. (1999) J. Neurosci. 19: 2500–2510.

[10] Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692.

[11] Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244.