Product Name: ERK2-2

Product Number: AB-NK056-3

Size:	25 µg		Price:	89.00
			$US

Target Full Name: Extracellular regulated protein-serine kinase 2 (p42 MAP kinase)

Target Alias: ERK; ERK-2; ERT1; Extracellular signal regulated kinase 2; Kinase ERK2; MAP kinase 2; MAP kinase isoform p42; MAPK; MAPK 2; PRKM2; p41mapk; CCDS13795.1; ENSG00000100030

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK056-3

Antibody Target Type: Pan-specific

Protein UniProt: P28482

Protein SigNET: P28482

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human ERK2 (MAPK1) sequence peptide Cat. No.: PE-01AXF99

Antibody Immunogen Sequence: HKNKVPWNRLFPNADC

Antibody Immunogen Description: Corresponds to amino acid residues H269 to D283; Pre-kinase last alpha-chain

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 1 µg/ml for immunoblotting

Antibody Species Reactivity: Human; Mouse

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-44 kDa.

Antibody Specificity: Medium

Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human ERK1 and ERK5.

Related Product 1: ERK2-2 blocking peptide

Related Product 2: ERK2-1 pan-specific antibody (Cat. No.: AB-NK056-2)

Related Product 3: ERK2-3 pan-specific antibody (Cat. No.: AB-NK056-4)

Related Product 4: ERK2-pT185+pY187 phosphosite-specific antibody (Cat. No.: AB-PK622)

Related Product 5: ERK1-pT202+pY204 phosphosite-specific antibody (Cat. No.: AB-PK621)

Related Product 6: ERK1-pY204 phosphosite-specific antibody (Cat. No.: AB-PK864)

Scientific Background: ERK2 (MAPK1) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It is involved in the MAP Kinase signalling pathway. The MAP kinase pathway can mediate cell adhesion, growth, survival, differentiation, meiosis, mitosis, and other post-mitotic functions. Phosphorylation of T185 and Y187 by MEK1 an MEK2 increases its phosphotransferase activity. It phosphorylates many diffrent transcription factors, such as ELK1. It acts as transcriptional repressor by binding directly to DNA. It is essential for cyclin D1 induction. MAPK1 phosphorylates BCL2, which contributes to cell survival, the suppression of the apoptotic effect of BAD and up-regulation of the antiapoptotic protein MCL-1. It regulates accumulation of p53 during DNA damage response. ERK2 appears to be a tumour requiring protein (TRP). The active form of the protein kinase normally acts to promote tumour cell proliferation. ERK2 has been linked with the Ewing's Family of tumours, which are rare cancers that are similar to medullablastoma and cerebral primitive neuroectodermal tumours. It is constitutively active in many human tumours, primarily due to altered Ras, Raf, EGFR or other upstream oncoproteins.

Figure 1. Dot blotting ERK2-2 antibody with recombinant purified proteins.